Abstract：Heavy panicle type is one of the main objectives of super rice breeding, and has the optimal combination for different yield factors per each plant, and is the basis for the high yield of unit area. Heavy panicle variant, Javanica 22 (Oryza sativa ssp. javanica), is a natural variation derived from Xiangdali (Oryza sativa ssp. javanica). Investigation in Javanica 22 physiological traits and genetic characteristics contributes to clarify its utilization value. In this study, the main physiological characters, the appearance quality, eating and cooking qualities (ECQs) of Javanica 22 and its wild type Xiangdali were compared. Indica or Japonica characteristic of Javanica 22 was identified by reported 20 pairs of typical SSR markers. According to the above result of identification, cluster analysis was carried out and SSR fingerprints of 4 varieties/lines were constructed. Glutinous property was experimented by molecular marker Wx M1. The results indicated that the weight of single panicle of Javanica 22 was 7.70 g, belonging to a variant of heavy panicle type. Compared with Xiangdali, the physiological traits of Javanica 22, especially the filled grain number, seed setting rate and single panicle weight were significantly improved (P<0.01), but 1000-grain weight was significantly decreased (P<0.01). The appearance quality and ECQs were also improved. Genetic analysis of Indica or Japonica characteristic showed that Javanica 22 had 8 pairs of markers with Japonica characteristics and 12 pairs of markers with Indica characteristics, belonged to mixed characteristics of Indica and Japonica but partial to Indica, yet its wild type Xiangdali belonging to mixed characteristics of Indica and Japonica but partial to Japonica. Cluster analysis also showed that Javanica 22 belonged to the same group with Jingnuo 6 (Oryza sativa ssp. indica) and its genetic similarity coefficient was 0.63; While its wild type belonged to the same group with Jingnuo 8 (Oryza sativa ssp. japonica), and its' genetic similarity coefficient was 0.81. Glutinous identification showed that the second exon of its waxy gene had nucleotides insertion of 23 bp, which was recessive wxwx genotype. Therefore, Javanica 22 was a typical glutinous rice with lower apparent amylose content (1.42%) than its wild-type, Xiangdali (3.80%). Multiple loci variation in Javanica 22 were found by comparing the DNA fingerprints of variant and wild type in this study. The heavy panicle variant Javanica 22 had more excellent physiological traits than its wild type and its genetic background had the characteristics of Indica and Japonica but partial to Indica, which could be used as an excellent germplasm in super rice breeding and as a bridge parent in hybridization of Indica and Japonica. The conclusion has definite theoretical significance in super rice breeding.
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