马铃薯炭疽病生防芽胞杆菌QB-2-7和BM-7的鉴定及其合成菌群的生防作用

doi:10.3969/j.issn.1674-7968.2025.11.009

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Abstract Potato anthracnose caused by Colletotrichum coccodes represents a significant phytopathological constraint in both potato (Solanum tuberosum) cultivation and postharvest storage. In this study, C. coccodes was used as the pathogen, and 2 endophytic bacteria (QB-2-7 and BM-7 ) with good bacteriostatic effect on C. coccodes preserved in the early stage of the laboratory were used to explore the control effect of its synthetic microbial community on potato anthracnose through in vivo and pot control experiments.Through comprehensive morphological characterization, physiological profiling, and molecular phylogenetic analysis, the isolates were taxonomically identified as Bacillus atrophaeus (QB-2-7) and B. safensis (BM-7), respectively. Biosafety assessment confirmed the non-hemolytic nature of both strains. Optimal consortium performance was achieved at a QB-2-7∶BM-7 ratio of 7∶3, demonstrating excellent microbial compatibility. Comparative analysis of different fermentation fractions revealed that the crude fermentation broth exhibited maximal antifungal activity (64.13% inhibition). In vivo evaluation of tuber protection efficacy demonstrated superior disease suppression in preventive applications (78.5% control efficiency) compared to both curative treatments and conventional azoxystrobin applications. The synthetic microbial community significantly enhanced host defense responses, as evidenced by elevated superoxide dismutase (SOD) and peroxidase (POD) activities, coupled with reduced polyphenol oxidase (PPO) activity and malondialdehyde (MDA) accumulation. Additional characterization revealed robust environmental tolerance of the fermentation products, including resistance to photodegradation, UV irradiation, and thermal stress, along with pronounced biofilm formation capacity. At the same time, the potato pot experiments showed that the synthetic microbial community had a significant prevention and control effect on potato anthracnose. These findings collectively suggest that the QB-2-7 and BM-7 synthetic microbial community consortium holds substantial promise as a biocontrol agent against potato anthracnose, providing both theoretical foundations and practical methodologies for developing microecological preparations targeting this economically important disease.

Key words： Potato Colletotrichum coccodes Synthetic microbial community Biological control

Received: 13 March 2025

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2025.11.009 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2025/V33/I11/2441

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