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| Effects of Different Pesticides on the Levels of Prostaglandin Synthetase, Isoprostaglandin and Reactive Oxygen Species in Adult Zebrafish (Danio rerio) |
| ZHOU Xin-Zong1*, WU Sheng-Gan2*, LI Shao-Bin1, CHANG Ju-Hua1** |
1 College of Life Sciences, Yangtze University, Jingzhou 434025, China;
2 Institute of Agricultural Product Quality Standard, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China |
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Abstract Prostaglandins (PGs) are important signaling molecules that play an important role in normal and pathophysiological processes. Many endocrine disruptors (EDCs) inhibit PG synthesis. However, there are few studies on the effect of pesticide EDCs on PG in zebrafish. The effects of 10 conventional pesticides on prostaglandin synthetase—cyclooxygenase (COX) (COX-1, COX-2) and isoprostaglandin (iso-PG) (8-iso PGF1α, 8-iso PGF2α, and iPF2α-Ⅵ), reactive oxygen species (ROS) and its related proteins (GST, CAT, SOD and POD) of adult zebrafish were detected by enzyme-related immunosorbent assay (ELISA). Then the correlation between different proteins and ROS or iPF2α - Ⅵ was analyzed. The ELISA results showed significant differences between male and female zebrafish treated with different pesticides, indicating that their effects on the levels of iPF2α-Ⅵ and ROS in zebrafish were sex-dependent. Correlation analysis showed that GST was significantly correlated with the levels of ROS (F=8.517; P=0.0092; R2=0.3212), iPF2α-Ⅵ was significantly positively correlated with ROS levels (F=75.51; P<0.0001; R2=0.8075), other proteins had no significant correlation with ROS. There was no significant correlation between each protein and iPF2α - Ⅵ . This study provides basic information for the screening and detection of pesticide endocrine disruptors.
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Received: 31 July 2024
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Corresponding Authors:
**Juhua1756@163.com
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| About author:: * These authors contributed equally to this work |
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