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| Genome-wide Identification of MaMKK Family in Banana (Musa spp.)and Their Expression Analysis in Response to Low Temperature Stress |
| LIN Wei1, WEI Mi2, LI Yue-Sen1, WU Shui-Jin1* |
1 Subtropical Agriculture Research Institute, Fujian Academy of Agricultural Sciences, Zhangzhou 363005, China;
2 Zhangzhou Institute of Agricultural Sciences, Zhangzhou 363005, China |
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Abstract The mitogen-activated protein kinase (MAPK) cascade is crucial to plant growth, development, and stress response, with MAPK kinase (MKK) serving a critical intermediary role in the cascade. Low temperature is one of the main reasons affecting banana (Musa spp.) yield. To explore the key genes for cold resistance in bananas, systematic evolutionary, gene structure assessment, chromosome localization andcollinearity analysis were conducted on the MaMKK gene family, clarifying the expression of the MaMKKgene family induced by low temperature in the 'Cavendish' (Musa AAA Cavendish) and 'Fenjiao' (MusaABB Pisang Awak). Subsequent analyses included sequence alignment and promoter element identification of the candidate genes. The result showed that A total of 13 members of the MaMKK gene family were identified and divided into 4 subclasses. Collinearity analysis showed that the members of the MaMKK gene family homologous had a strong connection, which had been purified and selected. After exposure to 7 ℃ , theexpression levels of MaMKK2a, MaMKK2b, and MaMKK5a significantly increased in both varieties, with agreater upregulation in 'Fenjiao' than in 'Cavendish'. However, the expression levels of MaMKK1, MaMKK2c, MaMKK3, and MaMKK5d showed a negative or unrelated relationship in the 2 varieties. Further analysis of these 7 candidate genes showed that MaMKK2a, MaMKK2b, and MaMKK5a responded more rapidly to low temperatures in 'Cavendish' than in 'Fenjiao'. The expression trends of MaMKK1, MaMKK2c, MaMKK3 and MaMKK5b showed significant differences within 48 h of low temperature treatment in 'Cavendish'/'Fenjiao'.The tissue expression results showed that there were significant differences in the tissue distribution of the 7 candidate genes in 'Cavendish'/'Fenjiao'. Analysis of the functional domains of 7 candidate genes revealed that they all contained phosphorylation targets for MEKK and MAPK, as well as multiple stress-related promotercis-acting elements. Among them, MaMKK2a and MaMKK5a contained low-temperature promoter cis-actingelements. The results indicated that the MaMKK gene in banana responded to low temperature stress, and the7 candidate genes screened may be related to the differences in cold resistance between 'Cavendish' and'Fenjiao'. This study provides a theoretical basis for developing cold-resistant banana varieties.
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Received: 18 June 2024
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Corresponding Authors:
*wusj305@163.com
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