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| Identification of Kenaf (Hibiscus cannabinus) WRKY Family Member and Analysis of Their Expression Under Cadmium Stress |
| LI Hui1,2, CHEN An-Guo2, TANG Hui-Juan2, LUAN Ming-Bao2,3,* |
1 College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde 415000, China;
2 Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China;
3 National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572000, China |
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Abstract It is a global challenge that the remediation of cadmium contaminated farmland. Kenaf (Hibiscus cannabinus) can be used to remediate Cd-contaminated farmland, as it has strong stress resistance. In order to explore the response patterns to Cd stress of HcWRKY gene family in kenaf, in this study, HMMER and BLAST software were used to identify HcWRKY family genes in genome-wide. The ExPasy website was used to obtain the molecular information of the HcWRKY members. The HcWRKY members were mapped to kenaf chromosomes using TBTOOLS v1.6 software. The results suggested that 33 HcWRKY family members were unequally scattered on 12 chromosomes. It was difference that the physical and chemical properties, such as the number of amino acids, molecular weight, and theoretical isoelectric point in each HcWRKY family member. Excepting the HcWRKY4-2 located in cytoplasm, the other HcWRKY family memebers were located in nucleus. Phylogenetic analysis showed that the 33 HcWRKY family members were divided into 4 groups. There was only HcWRKY member in Group Ⅱ and Group Ⅳ; while Groups Ⅰ and Ⅲ contained HcWRKY and AtWRKY proteins. qRT-PCR analysis indicated that 24 HcWRKY family members were induced by Cd stress. During the response to cadmium stress in kenaf, the expression levels of 22 HcWRKY family member genes were up-regulated, while the expression levels of 2 HcWRKY family member genes were down-regulated, which indicated that these HcWRKY genes improved greatly camdium tolerance of kenaf. These results provides basic information for further exploring the biological role of the HcWRKY gene family response to cadmium stress.
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Received: 25 September 2023
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Corresponding Authors:
* luanmingbao@caas.cn
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