红麻转录因子基因<em>HcNAC1</em>的分离及其在干旱胁迫下的表达模式

doi:10.3969/j.issn.1674-7968.2021.02.005

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Abstract Drought is one of the abiotic limiting factors that seriously affect the growth of crops. NAC (NAM, ATAF and CUC) transcription factor is one type of transcription factors in plants, it play an important role in regulating plant growth, development and response to abiotic stress. In order to study the regulation function of NAC transcription factor in drought stress of kenaf (Hibiscus cannabinus), a NAC like gene was isolated from kenaf transcriptome database, named HcNAC1 gene (GenBank No. MT799841). The coding sequence of HcNAC1 contained 1 044 bp, encoding 347 amino acids, protein molecular weight 38.77 kD, isoelectric point was 8.78. The HcNAC1 genes with NAM/NAC family conservative area, containing about 160 amino acid conservative in the protein N-terminal structural domain, with 2 nuclear localization signal (NLS) sequence. Phylogenetic tree analysis showed that kenaf HcNAC1 had the closest evolutionary relationship with Hibiscus syriacus. The expression level in different tissues and within 24 h of drought stress was detected by reverse transcription PCR (RT-PCR) and qRT-PCR, research results showed that HcNAC1 gene was expressed in root, stem, leaf, flower and fruit, HcNAC1 gene was expressed under PEG6000 stress and had most expression level at 12 h, and then the expression level gradually decreased. HcNAC1 played an important regulatory role in response to drought stress. The study explored the correlation between HcNAC1 expression and drought stress. and could provide a reference for further research on the molecular mechanism of HcNAC1 transcription factors involved in drought stress of kenaf.

Key words： Hibiscus cannabinus NAC transcription factor Drought stress Expression pattern analysis

Received: 22 April 2020 Published: 01 February 2021

ZTFLH:

S563.5

Corresponding Authors: * gyzhang2015@163.com

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	Articles by authors
	DENG Jie-Lou
	ZHANG Gao-Yang
	ZHANG Zong-Liang
	ZHANG Chao
	XU Jian-Tang
	QI Jian-Min
	WU Ying-Bao
	WANG Gang

Cite this article:

DENG Jie-Lou,ZHANG Gao-Yang,ZHANG Zong-Liang, et al. Isolation of HcNAC1 Transcription Factor Gene and Its Expression Patterns Under Drought Stress in Kenaf (Hibiscus cannabinus)[J]. 农业生物技术学报, 2021, 29(2): 251-257.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.02.005 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I2/251

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