UePep1对菰黑粉菌致病力的调控作用

doi:10.3969/j.issn.1674-7968.2024.07.014

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Abstract Ustilago esculenta can induce the formation of the enlarged fleshy stem of Zizania latifolia after obligate infection, and its pathogenicity is closely related to the phenotype and yield of the swollen stem called Jiaobai. Pep1 (protein essential during penetration 1), as a fungal effector, plays an important role in the pathogenicity of Ustilago fungi. In this study, UePep1 (GenBank No. OR088111), the homologous protein coding gene of Pep1 in U. esculenta, was cloned by BLASTP mapping and sequence analysis, with an ORF of 528 bp, encoding 175 amino acids and no intronic sequences. The deletion of UePep1 gene did not affect the haploid morphology, growth rate, mycelial formation and filamentous growth of U. esculenta in vitro. However, it significantly inhibited the invaded mycelial proliferation of the U. esculenta in vivo, and could not induce the formation of the enlarged fleshy stem. Further supplementation of UePep1 into the SG200ΔPep1 strain of Ustilago maydis partially restored the infectivity of the SG200ΔPep1 strain, indicating that UePep1 is a functional complementary protein of the Pep1 in the smut fungi. The results provide a reference and theoretical support for further understanding of the interaction mechanism between U. esculenta and Z. latifolia and the formation mechanism of enlarged fleshy stem.

Key words： Ustilago esculenta Protein essential during penetration 1 (Pep1) Zizania latifolia Pathogenicity

Received: 08 June 2023

ZTFLH:	S961.6
	S182
	S188
	Q81

Corresponding Authors: * zyfzjhzyh@163.com

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	Articles by authors
	HUANG Lin-Yu
	TANG Jin-Tian
	XIA Wen-Qiang
	CUI Hai-Feng
	YE Zi-Hong
	ZHANG Ya-Fen

Cite this article:

HUANG Lin-Yu,TANG Jin-Tian,XIA Wen-Qiang, et al. Regulation Effect of UePep1 in the Pathogenicity of Ustilago esculenta[J]. 农业生物技术学报, 2024, 32(7): 1614-1625.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2024.07.014 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2024/V32/I7/1614

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