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| Polymorphism of PRSS12 Gene and Its Association Analysis with Growth Traits in Sheep (Ovis aries) |
| XIONG Jin-Ke, BAO Jing-Jing, BI Ya-Zhen, HU Wen-Ping, ZHANG Li* |
| Institute of Animal Science, Chinese Academy of Agricultural Sciences/State Key Laboratory of Animal Biotech Breeding, Beijing 100193, China |
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Abstract The identification of sheep (Ovis aries) molecular markers related to growth traits is of great significance to improve the economic benefits of sheep industry. Serine protease 12 (PRSS12), also known as neurotrypsin, is specifically expressed in the central nervous system and is associated with a variety of neurological functions. In order to explore the polymorphism of PRSS12 gene and its correlation with the growth traits of Hu sheep and Ujumqin sheep, this study used gene chip technology to genotype 1 008 Hu sheep and 336 Ujimqin sheep, and conducted association analysis with growth traits to identify molecular markers related to sheep growth and development. The results of genotyping showed that two SNP loci were detected in Ujimqin sheep population, in which rs402399470G>A presented low polymorphism, while rs418440631G>A showed moderate polymorphism. And only one SNP locus, namely rs418440631G>A, was detected in Hu sheep population, which showed low polymorphism. The results of association analysis with growth traits showed that rs402399470G>A locus was significantly correlated with 4-month-old body weight and 6-month-old chest width (P<0.05), while rs418440631G>A locus was significantly correlated with 4-month-old chest circumference, tube circumference and 6-month-old chest circumference of Ujimqin sheep (P<0.05). The rs418440631G>A locus was extremely significantly correlated with birth weight and 5-month chest circumference of Hu sheep (P<0.01), and significantly correlated with 3-month tube circumference, 5-month body length and chest width of Hu sheep (P<0.05). The correlation coefficient between body weight and body size of Ujimqin sheep and Hu sheep was calculated by R language corrplot package, showing a significant positive correlation between weight and body size (P<0.05), with the highest correlation coefficient between weight and chest circumference. There was a significant positive correlation between body weight and body size, and the correlation coefficient between weight and chest circumference was the highest. In conclusion, the rs402399470G>A and rs418440631G>A loci of PRSS12 gene could be used as potential molecular genetic markers for the growth traits of sheep. This study provides a reference basis for mutton sheep breeding and selection.
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Received: 04 September 2023
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Corresponding Authors:
* zhangli07@caas.cn
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