Study on Thermotolerance Function and Regulatory Pathways of Heat Shock Transcription Factor ZmHsf06 in Maize (Zea mays)
LI Ran1,2,*, MA Zhen-Yu2,*, ZHANG Shi-Chang3, LI Guo-Liang2, MENG Xiang-Zhao2, DUAN Shuo-Nan2, LIU Zi-Hui2, LYU Ai-Zhi1, GUO Xiu-Lin2, ZHANG Hua-Ning2,**
1 College of Agriculture and Forestry Science and Technology, Hebei North University, Zhangjiakou 075000, China; 2 Institute of Biotechnology and Food Science/Hebei Key Laboratory of Plant Genetic Engineering, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China; 3 Wheat Engineering Research Center of Hebei Province, Shijiazhuang Academy of Agriculture and Forestry Sciences, Shijiazhuang 050041, China
Abstract Heat frequently affects yield and quality in different growth stages of maize (Zea mays). Heat shock transcription factor (Hsf) plays important roles in improving plant heat tolerance through regulating the expression of thermotolerance-related and activating multiple downstream pathways. Based on previous studies on characteristics and thermotolerance function of maize A1 subclass ZmHsf06, this study investigated its function and molecular mechanism in regulating thermotolerance through transformation in maize and chromatin immunoprecipitation-seq (ChIP-seq), and verified the protein interaction and transcriptional activation activity further. The results showed that ZmHsf06 had transcriptional activation activity and could interact with itself. Compared with the wild type, the thermotolerance of maize lines overexpressing ZmHsf06 were significantly improved. Analyzing the downstream target genes and high enrichment pathways of ZmHsf06 by ChIP-seq, a total of 2 218 differentially enriched genes were found under heat stress, including 1 169 genes up-regulated by ZmHsf06 specially, which were mainly enriched in electron carrier activity, photosynthesis, endoplasmic reticulum protein processing and heat shock response. The ZmHsf06 binding motif had heat shock elements (HSE), the binding elements of other transcription factors such as C2C2, TEAD and MADS and some unknown elements. Electrophoretic mobility shift assay (EMSA) showed that ZmHsf06 could bind to the heat shock elements located in the promoters of 3 target genes (Zm00001d000260, Zm00001d000272 and Zm00001d033987). These results will provide evidences for furthering the study of thermotolerance function and regulation mechanism of maize Hsf family.
LI Ran,MA Zhen-Yu,ZHANG Shi-Chang, et al. Study on Thermotolerance Function and Regulatory Pathways of Heat Shock Transcription Factor ZmHsf06 in Maize (Zea mays)[J]. 农业生物技术学报, 2024, 32(3): 505-517.
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