|
|
|
| Identification of Lipoxygenase Family Genes in Maize (Zea mays) and Expression Analysis Under Fusarium graminearum Infection and MeJA Treatment |
| LI Wei1, ZHANG Wei-Yu1, LIU Ying-Zi2, MA Wen-Xiao1, LI Zi-Yuan1, ZHANG Kang1, XING Ji-Hong1, CAO Hong-Zhe1,*, DONG Jin-Gao1,* |
1 State Key Laboratory of North China Crop Improvement and Regulation / Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding 071000, China;
2 Garden Management Centre of Hebei Province, Chengde 067000, China |
|
|
|
|
Abstract Lipoxygenase (LOX) is an important oxidoreductase in plants, which is widely involved in plant growth, development and response to biological and abiotic stresses. At present, the members of maize (Zea mays) LOX family and their roles in jasmonic acid (JA) signaling pathway are still not clear. In order to explore the function of LOX family genes in maize, bioinformatics methods were used to identify maize LOX family genes, and analyzed their phylogenetic evolution, conserved motifs, tissue expression specificity and expression patterns under biological and abiotic stresses. The expression of maize LOX family genes inoculated with Fusarium graminearum and induced by methyl jasmonate (MeJA) were detected by qRT-PCR. The results showed that maize genome contained 13 LOX family genes, which could be divided into 9-LOX, 13-LOX Type Ⅰ and 13-LOX Type Ⅱ, and all of them contained conservative lipoxygenase motif. The expression levels of ZmLOXs showed significant differences in different tissues or under different biological and abiotic stresses (P<0.05). After F. graminearum inoculation and MeJA treatment, the expression levels of 13 LOX family genes in maize were significantly up-regulated (P<0.05). These results suggested that maize LOX family genes might have important functions in maize growth and development and response to different biological and abiotic stresses. This study provides a reference for further understanding the function and mechanism of LOX family genes in maize.
|
|
Received: 14 April 2022
|
|
|
|
Corresponding Authors:
* caohongzhe1986@hotmail.com; dongjingao@126.com
|
|
|
|
[1] Avanci N C, Luche D D, Goldman G H, et al. 2010. Jasmo-nates are phytohormones with multiple functions, in-cluding plant defense and reproduction[J]. Genetics and Molecular Research, 9(1): 484-505.
[2] Bannenberg G, Martínez M, Hamberg M, et al. 2009. Diversi-ty of the enzymatic activity in the lipoxygenase gene family of Arabidopsis thaliana[J]. Lipids, 44(2): 85-95.
[3] Brash A R.1999. Lipoxygenases: Occurrence, functions, ca-talysis, and acquisition of substrate[J]. The Journal of Biological Chemistry, 274(34): 23679-23682.
[4] Cao S, Chen H, Zhang C, et al. 2016. Heterologous expres-sion and biochemical characterization of two lipoxygen-ases in oriental melon, Cucumis melo var. makuwa Maki-no[J]. PLOS ONE, 11(4): e0153801.
[5] Chen Z, Chen X, Yan H, et al. 2015. The lipoxygenase gene family in poplar: Identification, classification, and ex-pression in response to MeJA treatment[J]. PLOS ONE, 10(4): e0125526.
[6] Ding H, Lai J, Wu Q, et al. 2016. Jasmonate complements the function of Arabidopsis lipoxygenase 3 in salinity stress response[J]. Plant Science, 244: 1-7.
[7] Farmer E E, Gasperini D, Acosta I F.2014. The squeeze cell hypothesis for the activation of jasmonate synthesis in response to wounding[J]. The New Phytologist, 204(2):282-288.
[8] Feussner I, Wasternack C.2002. The lipoxygenase pathway[J]. Annual Review of Plant Biology, 53: 275-297.
[9] Gao X, Starr J, Göbel C, et al. 2008. Maize 9-lipoxygenase ZmLOX3 controls development, root-specific expres-sion of defense genes, and resistance to root-knot nema-todes[J]. Molecular Plant-Microbe Interactions, 21(1): 98-109.
[10] Hou Y, Meng K, Han Y, et al. 2015. The persimmon 9-lipoxy-genase gene DkLOX3 plays positive roles in both pro-moting senescence and enhancing tolerance to abiotic stress[J]. Frontiers in Plant Science, 6: 1073.
[11] Kolomiets M V, Hannapel D J, Chen H, et al. 2001. Lipoxy-genase is involved in the control of potato tuber develop-ment[J]. The Plant Cell, 13(3): 613-626.
[12] Liavonchanka A, Feussner I.2006. Lipoxygenases: occur-rence, functions and catalysis[J]. Journal of Plant Physi-ology, 163(3): 348-357.
[13] Liu F, Li H, Wu J, et al. 2021. Genome-wide identification and expression pattern analysis of lipoxygenase gene family in banana[J]. Scientific Reports, 11(1): 9948.
[14] Marla S S, Singh V K.2012. LOX genes in blast fungus (Mag-naporthe grisea) resistance in rice[J]. Functional & Inte-grative Genomics, 12(2): 265-275.
[15] Maschietto V, Marocco A, Malachova A, et al. 2015. Resis-tance to Fusarium verticillioides and fumonisin accumu-lation in maize inbred lines involves an earlier and en-hanced expression of lipoxygenase (LOX) genes[J]. Journal of Plant Physiology, 188: 9-18.
[16] Pathi K M, Rink P, Budhagatapalli N, et al. 2020. Engineering smut resistance in maize by site-directed mutagenesis of LIPOXYGENASE 3[J]. Frontiers in Plant Science, 11:543895.
[17] Sarde S J, Kumar A, Remme R N, et al. 2018. Genome-wide identification, classification and expression of lipoxy-genase gene family in pepper[J]. Plant Molecular Biolo-gy, 98(4): 375-387.
[18] Shaban M, Ahmed M M, Sun H, et al. 2018. Genome-wide identification of lipoxygenase gene family in cotton and functional characterization in response to abiotic stresses[J]. BMC Genomics, 19(1): 599-611.
[19] Shrestha K, Pant S, Huang Y.2021. Genome-wide identification and classification of lipoxygenase gene family and their roles in sorghum-aphid interaction[J]. Plant Molec-ular Biology, 105: 527-541.
[20] Sinha K, Kaur R, Singh N, et al. 2020. Mobilization of stor-age lipid reserve and expression analysis of lipase and li-poxygenase genes in rice (Oryza sativa var. Pusa Basma- ti 1) bran during germination[J]. Phytochemistry, 180:112538.
[21] Song H, Wang P, Li C, et al. 2016. Identification of lipoxygen-ase (LOX) genes from legumes and their responses in wild type and cultivated peanut upon Aspergillus flavus infection[J]. Scientific Reports, 6: 35245.
[22] Upadhyay R K, Mattoo A K.2018. Genome-wide identification of tomato (Solanum lycopersicum L.) lipoxygenas-es coupled with expression profiles during plant devel-opment and in response to methyl-jasmonate and wounding[J]. Journal of Plant Physiology, 231: 318-328.
[23] Wang J, Hu T, Wang W, et al. 2019. Bioinformatics analysis of the lipoxygenase gene family in radish (Raphanus sa- tivus) and functional characterization in response to abi-otic and biotic stresses[J]. International Journal of Mo-lecular Sciences, 20(23): 6095.
[24] Woldemariam M G, Ahern K, Jander G, et al. 2018. A role for 9-lipoxygenases in maize defense against insect herbivo-ry[J]. Plant Signaling & Behavior, 13(1): e1422462. |
|
|
|
