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Effects of Intermittent Fasting on Microbial Community Structure, Water Quality and Growth of Litopenaeus vannamei in a Biofloc System |
QU Yin, ZHANG Xiao-Dong, REN Gang, SHEN Wen-Ying* |
College of Life Sciences, Shaoxing University, Shaoxing 312000, China |
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Abstract Diet restriction has been used as a strategy to reduce the feed and labor cost in the aquaculture. The compensatory growth is widely present in mammals, fish and crustaceans. Based on full compensatory growth ability of Litopenaeus vannamei, the biofloc technology combined with diet restriction were applied to L. vannamei breeding. The effects of intermittent fasting on the microflora structure, water quality and growth parameters of L. vannamei in a biofloc system were investigated. The L. vannamei were randomly assigned into 4 groups (each with 3 replicates): Group 1 was the control group, normally fed with basal L. vannamei feed. Group 2 was the biological floc group, fed with L. vannamei feed and brown sugar (70% of the feed), and the C/N ratio was 12 in water. Group 3 was the probiotic and biological floc group, besides feed and brown sugar, Bacillus licheniformis (concentration was 1.2×105 CFU/mL in water) was also added; Group 4 was the intermittent starved group, starved for 2 d and re-feeding for 5 d. The results indicated the biological floc sedimentation in water showed an increasing trend. At the 4th week, the formation of biological floc in group 2, 3 and 4 were significantly higher than that in group 1 (P<0.05), respectively. The operational taxonomic units (OTU) of microflora in group 1, group 2, group 3 and group 4 were 3 501, 6 386, 5 387 and 6 577, with high throughput sequencing, respectively. The unique OTU numbers were 165, 463, 362 and 592, respectively, and 173 OTUs were shared by these 4 groups. The α diversity order of the bacterial community from high to low was: Group 4>group 2>group 3>group 1. The abundance analysis at genus level showed that the most dominant flora of group 1 was Georgenia (20.2%), followed by Hydrogenophaga (13.5%) and Pseudomonas (13.2%). In group 2, the highest abundance was Pseudomonas (39%), followed by Georgenia (9.1%) and Brevundimonas (7.0%). In group 3,the highest abundance was Brevundimonas (38.4%), followed by Microbacterium (5.7%) and Methyloversatilis (5.7%). In group 4, the highest abundance was Alishewanella (21.0%), followed by Pseudomonas (15.5%) and Exiguobacterium (6.8%). The concentration of ammonia nitrogen and nitrite nitrogen significantly decreased in groups 2, 3 and 4 (P<0.05). The feed conversion rate (FCR) of group 4 was significantly higher than 3 other groups (P<0.05). The results showed the biofloc technology based on compensatory growth could effectively increase the richness and diversity of microflora, optimize the composition of the microbial community, reduce the concentration of ammonia nitrogen and nitrite nitrogen in aquaculture water of L. vannamei, thereby to optimize aquaculture water quality, and effectively increase the feed conversion rate.The results were conducive to elucidating the mechanism of biofloc optimizing water quality, and provide experiental data for the application of the biofloc technology based on compensatory growth in L. vannamei culture.
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Received: 29 August 2022
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Corresponding Authors:
*zoology@usx.edu.cn.
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[1] 高戈, 朱开玲, 张庆起, 等. 2017. 一株功能益生菌的简易发酵及其在凡纳滨对虾生物絮团养殖中的应用[J]. 渔业科学进展, 38(3): 140-146. (Gao G, Zhu K L, Zhang Q Q, et al.2017. Simplified fermentation of a functional probiotics and the application in prawn (Litopenaeus vanname) bio-floc breeding[J]. Progress in Fishery Sciences, 38(3): 140-146.) [2] 葛红星, 李健, 陈萍, 等. 2014. 麦麸、蔗糖和芽孢杆菌发酵液对室内工厂化养殖凡纳滨对虾水质和生长的影响[J].中国渔业质量与标准, 3(4): 55-61. (Ge H X, Li J, Chen P, et a1.2014. Effects of the fermentation solution of wheat bran,cane sugar and Bacillus substilis on water quality and growth performance of cultured Penaeus vannamei in indoor industrial facilities[J]. Chinese Fishery Quality and Standards, 3(4): 55-62.) [3] 国家环境保护总局《水和废水监测分析方法》编委会. 2002.水和废水监测分析方法(增补版)4版[M]. 北京: 中国环境科学出版社, pp.4-10. (Editorial Board of Water and Wastewater Monitoring and Analysis Methods, State Environmental Protection Administration. 2002. Methods for monitoring and analysis of water and wastewater (Supplement) Version 4[M]. Beijing: China Environmental Science Press, pp.4-10. [4] 洪徐鹏, 万建军. 2013. 南美白对虾人工养殖中病害发生的原因及其防治对策[J]. 水产养殖, 9: 42-44. (Hong X P,Wan J J.2013. Causes and counter measures of diseases in artificial culture of Penaeus vannamei[J]. Journal of Aquaculture, 9: 42-44. ) [5] 李京昊, 成永旭, 王海锋, 等. 2019. 利用生物絮团技术对克氏原螯虾的养殖效果初探[J]. 水产学报, 43(4): 967-977. (Li J H, Cheng Y X, Wang H F,et al.A preliminary study on the feeding effect of the red swamp crayfish(Procambarus clakii) using biofloc technology[J]. Journal of Fisheries of China, 43(4): 967-977.) [6] 李晓梅, 郭体环. 2017. 生物絮团对凡纳滨对虾养殖过程中氨氮和亚硝酸氮含量的影响[J]. 渔业研究, 39(4): 283-286. (Li X M, Guo T H.2017. Impact of biological floc on ammonia nitrogen and nitrite nitrogen content in the process of Litopenaeus vannamei culture[J]. Journal of Fislieries Research, 39(4): 283-286.) [7] 林亮. 2005. 芽孢杆菌制剂对虾池微生物群落以及对虾肠道菌群的影响[D]. 硕士论文, 暨南大学, 导师: 林小涛, 李卓佳, pp. 20-30. (Lin L.2005. The effect of Bacillus probiotics on microbial communities and shrimp intestinal microflora[D]. Thesis for M.S., Jinan University, Supervisor: Lin X T, Li Z J, pp. 20-30.) [8] 林小涛, 潘剑雄, 许忠能, 等. 2008. 周期性断食对凡纳滨对虾摄食、生长和排粪的影响[J]. 水生生物学报, 32(3):403-407. (Lin X T, Pan J X, Xu Z N, et al.2008. Effect of periodic starvation on feeding, growth and defecation of Litopenaeus vannamei[J]. Acta Hydrobiologica Sinica, 32(3): 403-407.) [9] 刘克明, 尤宏争, 马林, 等. 2019. 不同碳源培养生物絮团对南美白对虾养殖影响试验[J]. 河北渔业, 04: 28-30. (Liu K M, You H Z, Ma L, et al.2019. Effects of biofloc cultured with different carbon sources on culture of Penaeus vannamei[J]. Hebei Fisheries, 4: 28-30.) [10] 刘强强, 陈旭, 谢家俊, 等. 2017. 饲料或养殖水体中添加地衣芽孢杆菌对凡纳滨对虾生长性能和免疫力的影响[J]. 动物营养学报, 29(8): 2808-2816. (Liu Q Q, Chen X, Xie J J, et al.2017. Effect of addingnBaclicus lincheniformi to diet or aquaulture water on growth performance and immunity of Litopenaeus vannamei[J]. Chinese Journal of Animal Nutrition, 29(8): 2808-2816.) [11] 柳泽锋. 2018. 碳源添加方式及卤虫混养比例对海水生物絮凝系统养殖凡纳滨对虾(Litopenaeus vannamei)的影响[D]. 硕士论文, 上海海洋大学, 导师: 罗国芝, pp. 22-35. (Liu Z F.2018. Effect of carbohy drates addition and Artemia mixed ratio on the culture of Litopenaeus vannamei in seawater biofloc technology-aquaculture system[D]. Thesis for M.S., Shanghai Ocean University, Supervisor: Luo G Z, pp. 22-35.) [12] 罗国芝, 朱泽闻, 潘云峰, 等. 2010. 生物絮凝技术在水产养殖中的应用[J].中国水产, 2: 62-63. (Luo G Z, Zhu Z W, Pan Y F, et al.2010. Application of biofloc technology in aquaculture[J]. China Fisheries, 2: 62-63.) [13] 饶毅, 徐先栋, 丁立云, 等. 2020. 不同饲料投喂量下生物絮团技术对草鱼养殖及水质的影响[J]. 湖南农业科学, 4:51-54; 57.(Rao Y, Xu X D, Ding L Y, et al. Effects of different feeding quantity on water quality and breeding of grass carp (Ctenopharyngodon idellus) in biofloc system[J]. Hunan Agricultural Sciences, 4: 51-54, 57.) [14] 沈文英, 任岗, 祝尧荣. 2012. 补偿生长对异育银鲫IGF-1,IGFBP-1水平及IGF-1, IGF-1R mRNA表达的影响[J],动物学研究, 33(3): 1-6. (Shen W Y, Ren G, Zhu Y R.2012. Effects of compensatory growth on the levels of IGF-1, IGFBP-1 and expressions of IGF-1 mRNA, IGF-1R mRNA in Carassius auratus gibelio[J]. Zoological Research, 33(3): 1-6.) [15] 万国强, 陈文斌, 潘鲁青. 2015. 不同C/N对生物絮团形成和凡纳滨对虾生长,养殖效果的影响[J]. 齐鲁渔业, 32(9): 1-4. (Wan G Q, Chen W B, Pan L Q.2015. Effects of different C/N on bioflocs formation and growth and culture effect of Litopenaeus vannamei[J]. Shandong Fisheries, 32(9): 1-4.) [16] 王超, 潘鲁青, 张开全. 2015. 生物絮团在凡纳滨对虾零水交换养殖系统中的应用研究[J]. 海洋湖沼通报, (2): 81-89. (Wang C, Pan L Q, Zhang K Q.2015. Research and Application of bioflocs in zero water exchange system for Litopenaeus vannamei aquaculture[J]. Transactions of Oceanology and Limnology, 2: 81-89.) [17] 王贤丰, 赵艳飞, 宋志飞, 等. 2017. 应用高通量测序技术分析拟穴青蟹肠道及其养殖环境菌群结构[J]. 中国水产科学, 24(06): 1245-1253. (Wang X F, Zhao Y F, Song Z F, et al.2017. Application of high-throughput sequencing techniques for analyzing bacterial communities in pond-raised mud crab (Scylla paramamosain) intestine and its aquaculture environment[J]. Journal of Fishery Sciences of China, 24(06): 1245-1253.) [18] 叶建勇, 单洪伟, 王贤丰, 等. 2016.添加甘蔗渣悬浮颗粒和芽孢杆菌对凡纳滨对虾生长和养殖环境的影响, 中国海洋大学学报, 46(4): 043-049. (Ye J Y, Shan H W, Wang X F, et al.2016. Effect of addition of suspended bagasse with Bacillus sp. on growth of Litopenaeus vannamei and environment of culture tank[J]. Periodical of Ocean University of China, 46(4): 043-049.) [19] 于赫男, 林小涛, 许忠能, 等. 2008. 凡纳滨对虾(Litopenaeus vannamei)继饥饿后恢复生长期间生化组成及能量收支的动态变化[J]. 海洋与湖沼, 02: 124-130. (Post-starvation recovery growth of Litopenaeus vannamei and variations in biochemical composition and energy budgeting[J]. Oceanologia et Limnologia Sinica, 02:124-130.) [20] 张欢欢, 王秀华, 李晨, 等. 2016.一株芽孢杆菌的分离鉴定及在生物絮团对虾养殖中的应用[J]. 渔业科学进展, 37(2): 111-118. (Zhang H H, Wang X H, Li C, et al.2016. Isolation and identification of a Bacillus sp.strain and its role in bioflocs for the shrimp culture system[J]. Progress in Fishery Sciences, 37(2): 111-118.) [21] 张哲, 杨章武, 葛辉, 等. 2019. 不同碳源对凡纳滨对虾育苗标粗水体生物絮团的结构、营养成分、细菌群落及其水质的影响[J]. 水产学报, 43(03): 639-649. (Zhang Z, Yang Z W, Ge H, et al.2019. Effects of different carbon sources on the biofloc formation,nutritional ingredients and bacterial community and quality in Litopenaeus vannamei culture tank[J]. Journal of Fisheries of China,43(3): 639-649.) [22] 周鲜娇, 邱德全. 2009. 亚硝酸氮和副溶血弧菌对凡纳滨对虾部分免疫指标的影响[J]. 水生态学杂志, 2(1): 49-53. (Zhou X J, Qiu D Q.2009. Effects of nitrite nitrogen and Vibrio parahaemolyticus on some immunity indicators of Litopenaeus vannamei[J]. Journal of Hydroecology, 2(1): 49-53.) [23] Andrew J, Beth L, Craig L, et al.2010. Suspended solids removal to improve shrimp (Litopenaeus vannamei) production and an evaluation of a plant-based feed in minimal-exchange, superintensive culture systems[J]. Aquaculture, 299: 89-98. [24] Avnimelech Y.1999. Carbon/nitrogen ratio as a control element in aquaculture systems[J]. Aquaculture, 176(3): 227-235. [25] Avnimelech Y.2007. Feeding with microbial flocs by tilaplain minimal discharge bioflocs technology ponds[J].Aquaculture, 264: 1-4. [26] Crab R, Avnimelech Y, Defoirdt T,et al.2007. Nitrogen removal techniques in aquaculture for a sustainable production[J]. Aquaculture, 270(1-4): 1-14. [27] Diana C, Wilson W, José M.2018. Quercetin influence in water quality and biochemical responses of shrimp Litopenaeus vannamei reared in Biofloc Technology System[J]. Aquaculture Research, 49: 11-18. [28] Foés G, Krummenauer D, Lara G, et al.2016. Long term and the compensatory growth of white shrimp Litopanaeus vannamei in aquaculture ponds[J]. Latin American Journal Aquatic Research, 44(3): 588-594. [29] Khanjani M H, Sharifinia M, Hajirezaee S.2020. Effects of different salinity levels on water quality, growth performance and body composition of pacific white shrimp (Litopenaeus vannamei Boone, 1931) cultured in a zero water exchange heterotrophic system[J]. Annals of Animal Science, 20(4): 442-452. [30] Kuhn D,Drahos D,Marsh L,et al.2010. Evaluation of nitrifying bacteria product to improve nitrification efficacy in recirculating aquaculture systems[J]. Aquaculture Enginering, 43(2): 78-82. [31] Lara G, Hostins B, Bezerra A, et al.2017. The effects of different feeding rates and re-feeding of Litopenaeus vannamei in a biofloc culture system[J]. Aquaculture Enginering, 77: 20-26. [32] Li,Z H, Song X, Wang J X, 2009. Effect of intermittent starvation on growth and some antioxidant indexes of Macrobrachium nipponense (De Haan)[J].Aquaculture Research, 40(5): 526-532. [33] Miglavs I, Jobling M.1989. Effects of feeding regime on food consumption, growth rates and tissue nucleic acids in juvenile Arctic charr, Salvelinm alpinus, with particular respect to compensatory growth[J]. Journal of Fish Biology, 34(6): 947-957. [34] Mohanty R, Mohapatra A.2017. Cyclic feed restriction on growth compensation of Penaeus monodon (Fabricius): science meets practice[J]. Indian Journal of Geo-Marine Sciences, 46(10): 2008-2016. [35] Ren W J, Li L, Dong S L, et al.2019. Effects of C/N ratio and light on ammonia nitrogen uptake in Litopenaeus vannamei culture tanks[J]. Aquaculture, 498: 123-131. [36] Rocha JV, Silva JF, Barros C, et al.2019. Compensatory growth and digestive enzyme activity of Litopenaeus vannamei submitted to feeding restriction in a biofloc system[J]. Aquaculture Research, 50(12): 3653-3662. [37] Singh R K, Balange A K.2007. Compensatory growth and changes in nutrient composition in post-larvae of giant prawn, Macrobrachium rosenbergii, following starvation[J]. Journal of Applied Aquaculture, 19(1): 39-49. [38] Stumpf L, Calvo NS, Díaz FC, et al.2011. Effect of intermittent feeding on growth in early juveniles of the crayfish Cherax quadricarinatus[J]. Aquaculture, 319(1-2): 98-104. [39] Tong R X, Chen W B, Pan L Q, et al.2020. Effects of feeding level and C/N ratio on water quality, growth performance, immune and antioxidant status of Litopenaeus vannamei in zero-water exchange bioflocs-based outdoor soil culture ponds[J]. Fish & shellfish immunology, 101:126-134. [40] Wu LX, Dong SL, Wang F, et al.2001a. The effect of previous feeding regimes on the compensatory growth response in Chinese shrimp, Fenneropenaeus chinensis[J]. Journal of Crustacean Biology, 21(3): 559-565. [41] Wu L X,Dong S L. 200lb.The effects of cyclic starvation and refeeding on the food consumption,growth, biochemical composition in Chinese shrimp, Fenneropenaeus chinensis (Osbeck, 1765) (Decapoda, Penaeidae).Crustacean, 74(11): 1225-1239. [42] Xia S Q,Wang F, Fu Y G, et al.et al.2005. Biodiversity analysis of microbial community in the chembio-flocculation treatment process[J]. Biotechnology and Bioengineering, 89(6): 656-659. [43] Xu W J, Timothy C, Tzachi M.2016. Effects of C/N ratio on biofioc development,water quality, and performance of Litopenaeus vannamei juveniles in a biofioc-based, high-density, zero-exchange, outdoor tank system[J]. Aquaculture, 5(453): 169-175. [44] Zhang P D, Zhang X M, Li J, et al.2010. Effect of refeeding on the growth and digestive enzyme activities of Fenneropenaeus chinensis juveniles exposed to different periods of food deprivation[J]. Aquaculture International, 18: 1191-1203. [45] Zhu Z M, Lin X T, Pan J X, 2016. Effect of cyclical feeding on compensatory growth, nitrogen and phosphorus budgets in juvenile Litopenaeus vannamei[J]. Aquaculture Research, 47: 283-289. |
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