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| Genetic Polymorphisms of FABP7 Gene and Their Correlative Analysis with Growth Traits in Coilia ectenes |
| YU Ai-Qing, SHI Yong-Hai*, YAN Yin-Long |
| Shanghai Fisheries Technology Extension Station, Shanghai Fisheries Research Institute, Shanghai 200433, China |
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Abstract As an important economic fish in China, the natural germplasm resources of Coilia ectenes are declining rapidly. Exploring the correlation between genetic polymorphisms of C. ectenes fatty acid-binding proteins 7 (CeFABP7) gene with the growth traits plays an important role in the breeding and improvement of growth traits of C. ectenes. Based on the transcriptome data of C. ectenes and gene cloning technology, the cDNA and DNA sequences of CeFABP7 were obtained. The SNPs of CeFABP7 gene was screened by DNA direct sequencing method using 20 individuals that selected from the F3 generation population of C. ectenes with extreme growth characteristics. Then 100 individuals that randomly selected from the same population of C.ectenes were genotyped by DNA direct sequencing or SnaPshot method. The relationship between these SNPs and growth traits of C. ectenes was analyzed by least squares method. The cDNA length of CeFABP7 was 1 295 bp, containing an open reading frame of 399 bp. The genomic DNA of CeFABP7 was 1 899 bp in length containing 3 introns and 4 exons. Six polymorphic sites were detected in 5′-UTR (2), intron 1 (2) and intron 3 (2). The locus g.276C>G was significantly associated with the body length, body height and body weigh traits of C. ectenes (P<0.05), the locus g.306A>C,g.747C>T and g.825C>T were significantly associated with body height and body weigh traits of C. ectenes (P<0.05), while the locus g.1447A>T and g.1532I>D were not significantly associated with 3 growth traits of C. ectenes (P>0.05). The result of population genetic analysis of cultured population of C. ectenes basing on 6 mutation locus showed that the average value of observed heterozygosity (Ho), expected heterozygosity (He) and polymorphism information content (PIC) were 0.257、0.339 and 0.275, respectively. The locus g.276C>G in the CeFABP7 gene was expected to be an important candidate molecular marker for eliminating inferior breeding parents of C. ectenes. This study has theoretical instructive significance for molecular marker assisted breeding in C. ectenes.
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Received: 07 July 2022
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Corresponding Authors:
* yonghais@163.com
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