山羊<em>IGF</em>-<em>1</em>基因的骨骼肌表达特性及其SNPs与生长性状的关联分析

doi:10.3969/j.issn.1674-7968.2019.12.010

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Abstract Major genes related to growth traits of goat (Capra hircus) were selected as marker assisted selection which can improve the accuracy of selection and accelerate breeding progress. The current study aimed to analyze gene expression characteristics of insulin-like growth factor-1 (IGF-1) gene in skeletal muscle, SNPs distribution and its association with growth traits of goats. qRT-PCR was used to analyze IGF-1 gene expression level in masseter, trapezius, semispinalis, arm triceps, extensor carpi radialis longus, longissimus dorsi, psoas, quadriceps femoris, biceps femoris of Fuqing goat and Nubia black goat. PCR sequencing and high-resolution melting (HRM) technologies were carried out to screen SNPs and analyzed their association with growth trait in Fuqing goat (n=124), Nubia black goat (n=152), Jianyang big ear goat (n=121), Daiyun goat (n=20). The results revealed that IGF-1 gene was expressed in 9 skeletal muscle tissues of Fuqing goat and Nubia black goat, and the expression level was different in skeletal muscle tissues and breeds. The expression level of IGF-1 gene in 9 skeletal muscle tissues of Fuqing goat was lower than that of Nubia black goat. The expression level of IGF-1 gene in masseter and quadriceps femoris was extremely significantly different between the 2 breeds (P<0.01), and the expression level of IGF-1 gene in arm triceps and trapezius and longissimus dorsi was significantly different between the 2 breeds (P<0.05). The exon sequence of IGF-1 gene was highly conserved, and no mutation was found in Fuqing goat and Nubia black goat. Most of the 3 SNPs of the 4 breeds showed moderate polymorphism distribution, PIC was 0.18~0.38; the 3 SNPs had obvious variety specificity on the growth traits of goats, and had significant influence on some growth traits of Jianyang big ear goat and Nubia black goat, but had little influence on the growth traits of Fuqing goat. The results of this study reveals the difference of IGF-1 gene expression in the skeletal muscles of Fuqing goat and Nubia black goat, and provides basic data for further exploring the mechanism of goat growth and development. The research results unveile that 3 SNPs within IGF-1 have strong effects on many growth traits of goats, which could be used as candidate markers for marker assisted selection of greater growth performance.

Key words： Insulin-like growth factor-1 gene (IGF-1) SNPs Growth traits Fuqing goat Nubia black goat

Received: 01 July 2019

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S828.2

Corresponding Authors: * seayuan521@163.com

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	LI Wen-Yang
	LIU Yuan
	WU Xian-Feng
	HUANG Qin-Lou

Cite this article:

LI Wen-Yang,LIU Yuan,WU Xian-Feng, et al. Skeletal Muscle Expression Characteristics of IGF-1 Gene and Its SNPs Association with Growth Traits in Goat (Capra hircus)[J]. 农业生物技术学报, 2019, 27(12): 2188-2197.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2019.12.010 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2019/V27/I12/2188

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