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| Association Analysis of the BMP2 Genotype with Sheep (Ovis aries) Tail Traits |
| LI Qing1, LU Zeng-Kui1, 2, JIN Mei-Lin1, QUAN Kai3, CHU Ming-Xing1, *, WEI Cai-Hong1, * |
1 Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2 College of Animal Science and Technology,Gansu Agriculture University, Lanzhou 730070, China;
3 College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450000, China |
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Abstract Bone morphogenetic protein 2 (BMP2) is named for its ability to initiate, promote and maintain the regeneration of cartilage and bone. In recent years, studies have found that BMP2 played an important role in promoting fat formation and was associated with the formation of sheep (Ovis aries) tail type. Therefore, BMP2 was chosen to be a novel candidate gene for the sheep tail traits in order to find new molecular markers for breeding. In the 533 sheep experiment group, 30 DNA samples of Hu sheep or Tibetan sheep with a fixed concentration were selected to construct the DNA pool, respectively, primers were designed to extend exons and 1 000 bp of upstream and downstream regulation regions. PCR products were tested and the objective strap-was sequenced. Sequenom Mass-array technology was used for genotype of detecting SNP. Haploview 4.1 was used to construct haplotypes and analyze linkage disequilibrium of the polymorphic loci. Association analysis between the SNPs in BMP2 gene and the tail traits were performed using SPSS 19.0 software. 7 SNPs were detected. rs402970343, rs594116558, rs160577302 and rs429072090 were related to tail length, tail width and tail perimeter. rs417201901 and rs402226298 were involved in tail width and tail perimeter. rs413373794 was only related to tail width. Meanwhile, the combined genotype of rs417201901-rs402970343 and rs413373794-rs402226298-rs429072090 correlation analysis revealed that: Individuals with CCGG and CCCCGG combined genotypes were significantly better than the other individuals on tail length, tail width and tail perimeter (P<0.05 or P<0.01). The results showed that the different SNPs and combined genotypes in BMP2 gene had effect on sheep tail traits with the SNPs being potentially valuable as genetic markers for breeding.
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Received: 11 July 2019
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Corresponding Authors:
mxchu@263.net; weicaihong@caas.cn
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