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| Effects of FADS2 Interference on Fatty Acid Composition of Mammary Epithelial Cells in Dairy Goats (Capra hircus) |
| WU Jiao1, HE Qiu-Ya1, LI Zhuang1, LI Cong1, WANG Hui2, SHI Huai-Ping1, LUO Jun1, * |
1 Shaanxi Provincial Key Laboratory of Agricultural Molecular Biology / College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China;
2 Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization / Sichuan Province and Ministry of Education, Southwest Minzu University, Chengdu 610041, China |
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Abstract Fatty acid desaturation 2 (FADS2), as a rate-limiting enzyme in the synthesis of polyunsaturated fatty acids (PUFAs）, such as arachidonic acid (AA) and docosahexaenoic acid (DHA), can dehydrogenate its substrate to form a double bond at the 6~7 position. In this study, FADS2 gene (GenBank No. MK292654), with 1 335 bp length of the CDS and encoding 444 amino acids, was cloned and had high homology with Ovis aries (XM_012146317.2) and Bos taurus (NM_001083444.1). Using siRNA technique in goat mammary epithelial cells (GMECs), the mRNA and protein levels of FADS2 decreased 60%~70% (P<0.01) and 15%~18% (P<0.05), respectively. Interfering FADS2 expression significantly up-regulated the expression of genes related to the synthesis of PUFAs, such as elongase of very long chain fatty acids 2 gene (ELOVL2) (P<0.01), ELOVL6 (P<0.01), fatty acid desaturation 1 gene (FADS1) (P<0.05); It also promoted the expression of sterol regulatory element-binding transcription protein gene (SREBP1a) (P<0.01), fatty acid synthase gene (FASN) (P<0.05), acetyl-CoA carboxylase gene (ACACA) (P<0.05), and stearoyl-CoA desaturase1 gene (SCD1) expression (P<0.05), respectively. In addition, interfering FADS2 expression could significantly decreased the proportion of AA and DHA (P<0.05) and restrained PUFAs synthesis (P<0.05). Furthermore, interfering FADS2 gene expression could significantly inhibit diacylglycerol acyltransferase1 (DGAT1) (P<0.01) and DGAT2 (P<0.05) gene expression to decrease triacylglyceride (TAG) content in GMECs (P<0.05). In conclusion, FADS2 gene plays an important role in regulating PUFAs synthesis and triglyceride metabolism in dairy goats, which provides experimental basis for the study of PUFAs metabolism in goat milk.
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Received: 18 February 2019
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Corresponding Authors:
luojun@nwsuaf.edu.cn
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