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| Changes of Gene Expression in Ovule of Chinese Fir (Cunninghamia lanceolata) Induced by Colchicine |
| PAN Yao-Yao, WU Bo-Hui, TONG Zai-Kang, LU Yong-Quan* |
| The State Key Laboratory of Subtropical Silviculture/College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China |
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Abstract Chinese fir (Cunninghamia lanceolata) has the characteristics of naked ovule, which provides favorable conditions for colchicine to enter the ovule quickly and improve induction efficiency. In order to study the induction effect of colchicine on ovule of Chinese fir, the gene expression changes were analyzed by transcriptome sequencing technique, and the expression characteristic of related genes were verified by qRT-PCR. The results showed that, for colchicine treatment group and control group, 6.39 and 6.96 Gb original data were obtained by transcriptome sequencing, respectively. A total of 60 790 unigenes were obtained after de novo assembly, and 1 430 differentially expressed genes were screened between the two groups, from which 4 spindle formation-related genes, 8 DNA replication-related genes, and 6 cell cycle-related genes were obtained and all showed significant down-regulated expression. qRT-PCR analysis showed that the gene expression trends of NEDD1 (neural precursor cell expressed, developmentally down-regulated gene 1), MCM3 (minichromosome maintenance complex component 3), MCM7, CDC6 (cell division cycle 6) and ATXR6 (trithorax-related protein 6) were consistent with those of transcriptome data. In this study, colchicine was directly acted on ovules and differentially expressed genes were screened, which provides basic information for further studying the molecular mechanism on polyploidy induction by colchicine.
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Received: 29 March 2019
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Corresponding Authors:
luyongquan@zafu.edu.cn
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