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| Association Analysis of the SINE Insertion Polymorphism in VRTN Gene with Growth and Reproductive Traits in Pig (Sus scrofa domesticus) |
| CHEN Wei, CHEN Cai, WANG Xiao-Yan, ZHANG Li, WANG Wei, SHEN Dan, WANG Sai-Sai, GAO Bo, SONG Cheng-Yi* |
| Tustitute of Animal Mobilome and Genome/College of Animal Science and Technology, Yangzhou University , Yangzhou 225009, China |
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Abstract The VRTN (vertebrae development associated) gene is an important regulatory gene for animal vertebrae development. Studies have shown that there is a short interspersed repeated sequence (SINE) insertion polymorphism in the VRTN gene of the porcine (Sus scrofa domesticus), which may be associated with the number of pig spines. In this study, the distribution of SINE insertion polymorphism in different pig breeds and its effects on growth and reproduction traits of pigs were studied by PCR detection, polymorphism correlation and bioinformatics analysis. The results showed that the frequency of SINE+ in introduced breeds was higher than that of cultivated pig breeds and Chinese local pig breeds. The age at 100 kg body weight of SINE+/+ pigs of Yorkshire was significantly higher than that of SINE-/- pigs (P<0.05), and the SINE+/+ pigs loin eye muscle area was significantly lower than that of SINE-/- pigs (P<0.05). There was no significant association between reproductive traits such as live litter size and SINE insertion polymorphism. SINE and VRTN genes were not found to form a fusion transcript after comparison with expressed sequence tag (EST). This study demonstrated that SINE insertion polymorphism in VRTN gene may be associated with pig growth rate and lean meat rate, which provided a reference for the application of this molecular marker in pig molecular breeding.
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Received: 12 September 2018
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Corresponding Authors:
* cysong@yzu.edu.cn
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