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| Genetic Polymorphism of the TNNI3 and MOGAT2 Genes and Its Association Analysis with Fat Deposition Traits in Hu Sheep (Ovis aries) |
| ZHOU Ying-Hui1, WANG Wei-Min2, TIAN Hui-Bin2, XU Quan-Zhong2, MA Zong-Wu2, YANG Xiao-Bin2, ZHANG Jian1, CAO Pei-Liang1, CAI Tong1, MA Bo1, ZHANG Xiao-Xue1,* |
1 College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2 College of Grassland Agricultural Science and Technology, Lanzhou University, Lanzhou 730000, China |
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Abstract Troponin I3 (TNNI3), which regulates myocardial contraction by inhibiting actin-myosin interaction and serves as a core biomarker for the clinical diagnosis of myocardial infarction; monoacylglycerol O-acyltransferase 2 (MOGAT2) primarily catalyzes the synthesis of diacylglycerol from monoacylglycerol and fatty acids in the small intestine, playing a key role in dietary fat absorption and re-esterification. To investigate the association between SNP in the TNNI3 and MOGAT2 genes and fat deposition traits in Hu sheep (Ovis aries), a total of 1 029 male Hu sheep with clear pedigrees, complete phenotypic records, and healthy conditions were selected as experimental subjects in this study. Polymorphic loci of the TNNI3 and MOGAT2 genes were detected using PCR amplification and MassARRAY® SNP genotyping technology. A general linear model was used for association analysis of fat deposition-related traits, and the tissue expression patterns of the 2 genes were further determined. The results revealed 2 polymorphic sites: TNNI3 g.66954963 T>G, located in the first intron of TNNI3 gene, and MOGAT2 g.58004696 A>G, located in the second intron of MOGAT2 gene. Association analysis indicated that the TNNI3 g.66954963 T>G polymorphism was significantly associated with caudal fat weight, perirenal fat weight, and mesenteric fat weight (P<0.05). The MOGAT2 g.58004696 A>G polymorphism was significantly associated with perirenal fat weight, relative perirenal fat weight, mesenteric fat weight, and relative mesenteric fat weight (P<0.05). The combined genotype GGTNNI3-GGMOGAT2 showed significantly lower values for caudal fat weight, perirenal fat weight, relative perirenal fat weight, mesenteric fat weight, and relative mesenteric fat weight compared to the TTTNNI3-GAMOGAT2 genotype group (P<0.05). RT-qPCR analysis demonstrated that high expression of the TNNI3 gene in perirenal and mesenteric fat, while the MOGAT2 gene was highly expressed in caudal fat. In conclusion, the TNNI3 and MOGAT2 genes played important roles in fat deposition in Hu sheep. This study provides potential molecular markers for molecular-assisted breeding.
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Received: 30 September 2025
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Corresponding Authors:
*zhangxx@gsau.edu.cn
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[1] 陈志蓉. 2019. 基于脂质代谢调控研究苦味中药黄连有效成分小檗碱逐瘀作用[D]. 博士学位论文, 北京中医药大学, 导师:孙建宁, pp. 99-128.
(Chen Z R.2019. Study on the blood stasis removing effects of berberine, an active ingredient of the bitter chinese herbal medicine coptis, based on lipid metabolism regulation[D]. Thesis for Ph.D., Beijing University of Chinese Medicine, Supervisor: Sun J N, pp. 99-128.)
[2] 马现永, 郑春田, 胡友军, 等. 2018. 慢型骨骼肌肌钙蛋白的调控对肥育猪肌内脂肪沉积、肉品质影响及分子机理[Z]. 广东省农业科学院动物科学研究所.
(Ma X Y, Zheng C T, Hu Y J, et al.2018. Regulation of slow skeletal muscle troponin on intramuscular fat deposition and meat quality in finishing pigs: Effects and molecular mechanisms[Z]. Institute of Animal Science, Guangdong Academy of Agricultural Sciences.
[3] 苏睿, 林峻, 陈鲤群, 等. 2019. 高通量自动化SNP检测技术研究进展[J]. 中国细胞生物学学报, 41(7): 1412-1422.
(Su R, Lin J, Chen L Q, et al.2019. Research progress on high-throughput automated SNP detection technology[J]. Chinese Journal of Cell Biology, 41(7): 1412-1422.
[4] 薛丽娜, 罗惠娣, 周胜花, 等. 2020. 绵羊MC1R和AGOUTI基因多态性与毛色性状的关联分析[J]. 山西农业大学学报(自然科学版), 40(06): 107-113.
(Xue L N, Luo H D, Zhou S H, et al.2020. Polymorphism of MC1R and AGOUTI genes and their association with coat colortraits in sheep[J]. Journal of Shanxi Agricultural University (Natural Science Edition), 40(06): 107-113.
[5] 杨华. 2009. 猪肌钙蛋白Ⅰ基因家族的基因结构、表达谱和多态性分析[D]. 硕士学位论文, 华中农业大学, 导师: 左波, pp. 52-58
(Yang H.2009. Analysis of gene structure, expression profile and polymorphism of porcine troponinⅠ gene family[D]. Thesis for M.S., Huazhong Agricultural University, Supervisor: Zuo B, pp. 52-58.)
[6] 杨少滢, 刘宇, 赵文军, 等. 2022. 沃金黑牛MOGAT2基因多态性及其与生长性状的关联分析[J].吉林畜牧兽医, 43(11):3-4.
(Yang S Y, Liu Y, Zhao W J, et al.2022. Polymorphism of MOGAT2 gene in Wagin Black Bull and its association with growth traits[J]. Jilin Animal and Veterinary Science, 43(11): 3-4.
[7] Armani M, Tangrea M A, Shapiro B, et al.2011. Quantifying mRNA levels across tissue sections with 2D-RT-qPCR[J]. Analytical and Bioanalytical Chemistry, 400(10): 3383-3393.
[8] Bartoszewski R A, Jablonsky M, Bartoszewska S, et al.2010. A synonymous single nucleotide polymorphism in DeltaF508 CFTR alters the secondary structure of the mRNA and the expression of the mutant protein[J]. Journal of Biological Chemistry, 285: 28741-28748.
[9] Cartegni L, Chew S L, Krainer A R, et al.2002. Listening to silence and understanding nonsense: Exonic mutations that affect splicing[J]. Nature Reviews Genetics, 3(4): 285-298.
[10] Chai W, Qu H, Ma Q, et al.2023. RNA-seq analysis identifies differentially expressed gene in different types of donkey skeletal muscles[J]. Animal Biotechnology, 34(5): 1786-1795.
[11] Chen C H, Li B J, Gu X H, et al.2019. Marker-assisted selec‐tion of YY supermales from a genetically improvedfarmed tilapia-derived strain[J]. Zoological Research, 40(2): 108-112.
[12] Dementieva N V, Shcherbakov Y S, Tyshchenko V I, et al.2022. Comparative analysis of molecular RFLP and SNP markers in assessing and understanding the genetic diversity of various chicken breeds[J]. Genes (Basel), 13(10): 1876.
[13] Drummond D, Wilke C O.2008. Mistranslation-induced protein misfolding as a dominant constraint on coding-sequence evolution[J]. Cell, 134: 341-352.
[14] Duan J, Wainwright M S, Comeron J M, et al.2003. Synonymous mutations in the human dopamine receptor D2 (DRD2) affect mRNA stability and synthesis of the receptor[J]. Human Molecular Genetics, 12: 205-216.
[15] EEr H H, Ma L N, Xie X L, et al.2020. Genetic polymorphism association analysis of SNPs on the species conservation genes of Tan sheep and Hu sheep[J]. Tropical Animal Health and Production, 52(3): 915-926.
[16] Gao Y, Nelson DW, Banh T, et al.2013. Intestine-specific expression of MOGAT2 partially restores metabolic efficiency in Mogat2-deficient mice[J]. Journal of Lipid Research, 54: 1644-1652.
[17] Goodman D B, Church G M, Kosuri S, et al.2013. Causes and effects of N-terminal codon bias in bacterial genes[J]. Science, 342: 475-479.
[18] Guo L, Su Y, Chen C, et al.2023. The TNNI3 p.R186Q mutation is responsible for hypertrophic cardiomyopathy via promoting FASN-stimulated abnormal fatty acid metabolism[J]. The Journal of Cardiovascular Aging, 3: 6.
[19] Ioannou G N, Van Rooyen D M, Savard C, et al.2015. Cholesterol-lowering drugs cause dissolution of cholesterol crystals and disperse Kupffer cell crown-like structures during resolution of NASH[J]. Journal of Lipid Research, 56(2): 277-285.
[20] Jin M L, Li T T, Sun D X, et al.2023. Research progress of epigenetic regulation in fat deposition mechanism of livestock and poultry[J]. Acta Veterinaria Et Zootechnica Sinica, 54(03): 855-867.
[21] Kimchi-Sarfaty C, Oh J M, Kim I W, et al.2007. A 'silent' polymorphism in the MDR1 gene changes substrate specificity[J]. Science, 315: 525-528.
[22] Kushanov F N, Turaev O S, Ernazarova D K, et al.2021. Genetic diversity, QTL mapping, and marker-assisted selection technology in cotton (Gossypium spp.)[J]. Frontiersin Plant Science, 12: 779386.
[23] Li Q, Li J, Yu C P, et al.2021. Synonymous mutations that regulate translation speed might play a non-negligible role in liver cancer development[J]. British Medical Council Cancer, 21(1): 388.
[24] Li T, Xing F, Zhang N, et al.2024. Genome-wide association analysis of growth traits in Hu Sheep[J]. Genes (Basel), 15(12): 1637.
[25] Li X, Yang J, Shen M, et al.2020. Whole-genome resequencing of wild and domestic sheep identifies genes associated with morphological and agronomic traits[J]. Nature Communications, 11: 2815.
[26] Liss K H H, Lutkewitte A J, Pietka T, et al.2018. Metabolic importance of adipose tissue monoacylglycerol acyltransferase 1 in mice and humans[J]. Journal of Lipid Research, 59(9): 1630-1639.
[27] Liu M, Huang C, Dai R, et al.2022. Copy number variations in the MICALL2 and MOGAT2 genes are associated with ashidan yak growth traits[J]. Animals (Basel), 12(20): 2779.
[28] Niersch J, Vega-Rubin-De-Celis S, Bazarna A, et al.2021. A BAP1 synonymous mutation results in exon skipping, loss of function and worse patient prognosis[J]. iScience, 24(3): 102173.
[29] Parmley J L, Chamary J V, Hurst L D.2006. Evidence for purifying selection against synonymous mutations in mammalian exonic splicing enhancers[J]. Molecular Biology and Evolution, 23: 301-309.
[30] Sauna Z E, Kimchi-Sarfaty C.2011. Understanding the contribution of synonymous mutations to human disease[J]. Nature Reviews Genetics, 12: 683-691.
[31] Sheng J J, Jin J P.2016. TNNI1, TNNI2 and TNNI3: Evolution, regulation, and protein structure-function relationships[J]. Gene, 576(1 Pt 3): 385-394.
[32] Viguerie N, Montastier E, Maoret J J, et al.2012. Determinants of human adipose tissue gene expression: Impact of diet, sex, metabolic status, and cis genetic regulation[J]. Public Library of Science Genetics, 8(9): e1002959.
[33] Wang W, Liu S, Li F, et al.2015. Polymorphisms of the ovine BMPR-IB, BMP-15 and FSHR and their associations with litter size in two chinese indigenous sheep breeds[J]. International Journal of Molecular Sciences, 16: 11385-11397.
[34] Wang Z, Guo Y, Liu S, et al.2021. Genome-wide assessment characteristics of genes overlapping copy number variation regions in duroc purebred population[J]. Frontiers in Genetics, 12: 753748.
[35] Yang X, Cheng J, Xu D, et al.2025. Differences in production performance, fore-digestive tract microbiota, and expression levels of nutrient transporters of Hu sheep with different feed conversion ratio[J]. Microbiology Spectrum, 13(6): e0142324.
[36] Zhao L, Li F, Yuan L, et al.2022. Expression of ovine CTNNA3 and CAP2 genes and their association with growth traits[J]. Gene, 807: 145949.
[37] Zhao L M, Li F D, Liu T, et al.2021. Ovine ELOVL5 and FASN genes polymorphisms and their correlations with sheep tail fat deposition[J]. Gene, 807: 145954.
[38] Zheng C, Zhong Y, Zhang P, et al.2024. Dynamic transcriptome profiles of skeletal muscle growth and development in Shaziling and Yorkshire pigs using RNA-sequencing[J]. Journal of the Science of Food and Agriculture, 104(12): 7301-7314. |
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