小麦茎秆水溶性碳水化合物含量主效QTL <em>QWSC.caas</em>-<em>7DS</em>精细定位

doi:10.3969/j.issn.1674-7968.2026.05.001

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Abstract Stem-reserved water-soluble carbohydrates (WSC) serve as a crucial carbon source for grain filling in wheat (Triticum aestivum). Enhancing stem WSC content is a key strategy for achieving high and stable grain yield. The major-effect QTL QWSC.caas-7DS, which regulates stem WSC content at 10 d after flowering (WSC10), was previously identified in the 'Yangmai 16' (YM16)/'Zhongmai 895' (ZM895) doubled haploid (DH) population, with physical interval of 11.7 Mb. To fine-map this QTL, identify the candidate genes, and develop molecular markers, a cross XY015/XY102 was made with 2 DH lines XY015 and XY102 that contrast in genotype at QWSC.caas-7DS but shared identical genotypes at other known WSC loci. Subsequently, F₂, F_2:3, and F_2:4 segregating populations were constructed. Based on whole-genome resequencing data of YM16 and ZM895, 20 kompetitive allele-specific PCR (KASP) markers were successfully developed within the target interval, and a high-density genetic map was constructed using 755 F₂ individuals. The homozygous F_2:4 lines derived from 35 recombinant F₂ plants were used for phenotyping of WSC10 in 3 environments; in combination with the genotypic data of these lines, QWSC.caas-7DS was finely mapped to the 66.44~69.14 Mb interval on chromosome 7DS. Comprehensive analysis of genomic resequencing data of YM16 and ZM895, along with annotations and expression profiles of the genes in the QTL interval, 6 genes were preliminarily presumed as the candidate genes for QWSC.caas-7DS. According to the gene expression profile database, Vrn-D3 (gene ID: TraesCS7D01G111600), one of the candidate genes, showed high expression levels in stem and leaf tissues, and it may regulate stem WSC and thousand-kernel weight (TKW) by modulating carbon balance between source and sink tissues. Vrn-D3 was cloned from YM16 and ZM895, and a G base "insertion/deletion" variation located in the third exon was identified. A molecular marker, Vrn-D3M, was developed for genotyping this variation and was validated in 157 wheat varieties from the Yellow and Huai Valleys Winter Wheat Zone. The results indicated that Vrn-D3M could be effectively used for molecular breeding targeting for improvement of WSC10 and TKW. The findings of this study lay a solid foundation for the final cloning and functional study of QWSC.caas-7DS, and also provide an efficient molecular tool for breeding applications.

Key words： Wheat Water-soluble carbohydrate Thousand-kernel weight Fine mapping QWSC.caas-7DS Vrn-D3

Received: 14 January 2026

ZTFLH:

S512.1

Corresponding Authors: ^*fuluping@yzu.edu.cn; lijihuyantai@163.com; taoli@yzu.edu.cn

About author:: ^#These authors contributed equally to this work

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	FU Lu-Ping
	WANG Guang-Liang
	XUE Bo-Yang
	ZHU Xue-Yuan
	MENG Heng-Yu
	LIU Bing-Liang
	ZHAO Ren-Hui
	YANG Zi-Bo
	ZHANG Yong
	REN Yan
	LIU Jin-Dong
	Li Tao
	Li Ji-Hu

Cite this article:

FU Lu-Ping,WANG Guang-Liang,XUE Bo-Yang, et al. Fine Mapping of a Major QTL QWSC.caas-7DS for Stem Water-soluble Carbohydrate Content in Wheat (Triticum aestivum)[J]. 农业生物技术学报, 2026, 34(5): 913-930.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2026.05.001 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2026/V34/I5/913

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