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Cloning and Functional Analysis of GmSDIR1 Gene in Soybean (Glycine max) |
ZHU Gui-Shuang1,3, ZHAO Ying2, WANG Xiao-Dong1, ZHANG Wei-Wei1, XIANG Dian-Jun1,*, LI Zhi-Gang1,* |
1 Agricultural College, Inner Mongolia University for Nationalities, Tongliao 028000, China; 2 Tongliao Agriculture and Animal Husbandry Development Center, Tongliao 028000, China; 3 Tongliao Academy of Agricultural and Animal Husbandry Sciences, Tongliao 028015, China |
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Abstract Salt- and drought-induced ring finger protein (SDIR) plays an important role in abscisic acid (ABA)-mediated plant stress response, and SDIR is rarely reported in soybean (Glycine max). In this study, the candidate gene GmSDIR1 was screened and cloned from soybean by co-expression analysis of transcriptome sequencing data and weighted gene co-expression network analysis (WGCNA). It was located on chromosome 11, with a full-length coding region of 825 bp, belonging to C3H2C3 type RING finger protein. Subcellular localization showed that GmSDIR1 protein was localized in the nucleus. The expression pattern analysis showed that GmSDIR1 gene was expressed in true leaf, cotyledon and stem tissues of soybean and had spatial and temporal expression specificity. High salt (NaCl), drought (PEG6000), abscisic acid (ABA) and low temperature (4 ℃) stress treatments could significantly induce the expression of GmSDIR1 gene.Phenotypic analysis showed that tobacco (Nicotiana tobacum) overexpressing GmSDIR1 gene was tolerant to high salt stress, and the heterologous expression of GmSDIR1 promoted seed germination, root length elongation and fresh weight increase of seedlings, and improved the antioxidant capacity of transgenic tobacco. The results of qRT-PCR showed that the expression of GmSDIR1 in transgenic tobacco was significantly activated under high salt stress, which indicated that GmSDIR1 might be involved in the regulation of plant response to salt stress. This study provides an important experimental basis for further analysis of the molecular mechanism of soybean GmSDIR1 gene regulating plant high salt stress response.
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Received: 11 December 2024
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Corresponding Authors:
*Xiangdianjun00@126.com; 13948651158@126.com
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[1] 陈刘平. 2023. 小麦TaRING-H2和TaLHC基因家族鉴定及TaSDIR1和TaLHC86的耐盐性分析[D]. 博士学位论文, 西北农林科技大学, 导师: 李学军, pp. 43-46. (Chen L P.2023. Identification of TaRING-H2 and TaLHC gene families in wheat and salt tolerance analysis of TaSDIR1 and TaLHC86[D]. Thesis for Ph. D., Northwest A & F University, Supervisor: Li X J, pp. 43-46.) [2] 王营. 2020. 过表达细叶百合LpNAC5和LpNAC13转基因烟草的抗逆性研究[D]. 硕士学位论文, 东北林业大学, 导师: 张艳妮, pp. 55-60. (Wang Y.2020. Study on the stress resistance of transgenic tobacco overexpressing LpNAC5 and LpNAC13 in Lilium pumilum[D]. Thesis for M. S., Northeast Forestry University, Supervisor: Zhang Y N, pp. 55-60.) [3] Baek W, Lim C W, Lee S C.2021. Pepper E3 ligase CaAIRE1 promotes ABA sensitivity and drought tolerance by degradation of protein phosphatase CaAITP1[J]. Journal of Experimental Botany, 72(12): 4520-4534. [4] Davey M W, Stals E, Panis B, et al.2005. High-throughput determination of malondialdehyde in plant tissues[J]. Analytical Biochemistry, 347(2): 201-207. [5] Gao T, Wu Y, Zhang Y, et al.2011. OsSDIR1 overexpression greatly improves drought tolerance in transgenic rice[J]. Plant Molecular Biology, 76: 145-156. [6] Joo H, Lim C W, Lee S C.2020. The pepper RING‐type E3 ligase, CaATIR1, positively regulates abscisic acid signalling and drought response by modulating the stability of CaATBZ1[J]. Plant, Cell & Environment, 43(8): 1911-1924. [7] Kelley D R, Estelle M.2012. Ubiquitin-mediated control of plant hormone signaling[J]. Plant Physiology, 160(1): 47-55. [8] Komander D, Rape M.2012. The ubiquitin code[J]. Annual Review of Lochemistry, 81(1): 203-229. [9] Li Q, Serio R J, Schofield A, et al.2020. Arabidopsis RING‐type E3 ubiquitin ligase XBAT35.2 promotes proteasome‐dependent degradation of ACD11 to attenuate abiotic stress tolerance[J]. The Plant Journal, 104(6): 1712-1723. [10] Li R, Wang W, Wang W, et al.2015. Overexpression of a cysteine proteinase inhibitor gene from Jatropha curcas confers enhanced tolerance to salinity stress[J]. Electronic Journal of Biotechnology, 18(5): 368-375. [11] Liu S, Zenda T, Dong A, et al.2021. Global transcriptome and weighted gene co-expression network analyses of growth-stage-specific drought stress responses in maize[J]. Frontiers in Genetics, 12: 645443. [12] Liu X, Jing R, Du L.2025. Genome-wide characterization of U-box family and their expression profiling with abiotic stress treatment in Populus alba[J]. Acta Physiologiae Plantarum, 47(4): 47. [13] Lyzenga W J, Stone S L.2012. Abiotic stress tolerance mediated by protein ubiquitination[J]. Journal of Experimental Botany, 63(2): 599-616. [14] Mallick N, Mohn F H.2000. Reactive oxygen species: Response of algal cells[J]. Journal of Plant Physiology, 157(2): 183-193. [15] Meng Y, Lv Q, Li L, et al.2024. E3 ubiquitin ligase TaSDIR1‐4A activates membrane‐bound transcription factor TaWRKY29 to positively regulate drought resistance[J]. Plant Biotechnology Journal, 22(4): 987-1000. [16] Mittler R, Vanderauwera S, Gollery M, et al.2004. Reactive oxygen gene network of plants[J]. Trends in Plant Science, 9(10): 490-498. [17] Moon J, Parry G, Estelle M.2004. The ubiquitin-proteasome pathway and plant development[J]. The Plant Cell, 16(12): 3181-3195. [18] Qin F, Sakuma Y, Tran L S P, et al.2008. Arabidopsis DREB2A-interacting proteins function as RING E3 ligases and negatively regulate plant drought stress-responsive gene expression[J]. The Plant Cell, 20(6): 1693-1707. [19] Santner A, Estelle M.2010. The ubiquitin‐proteasome system regulates plant hormone signaling[J]. The Plant Journal, 61(6): 1029-1040. [20] Sharma M K, Solanke A U, Jani D, et al.2009. A simple and efficient Agrobacterium-mediated procedure for transformation of tomato[J]. Journal of Biosciences, 34: 423-433. [21] Smalle J, Vierstra R D.2004. The ubiquitin 26S proteasome proteolytic pathway[J]. The Annual Review of Plant Biology, 55(1): 555-590. [22] Stone S L, Hauksdóttir H, Troy A, et al.2005. Functional analysis of the RING-type ubiquitin ligase family of Arabidopsis[J]. Plant Physiology, 137(1): 13-30. [23] Tak H, Mhatre M.2013. Molecular characterization of VvSDIR1 from Vitis vinifera and its functional analysis by heterologous expression in Nicotiana tabacum[J]. Protoplasma, 250: 565-576. [24] Vierstra R D.2012. The expanding universe of ubiquitin and ubiquitin-like modifiers[J]. Plant Physiology, 160(1): 2-14. [25] Xia Z, Liu Q, Wu J, et al.2012. ZmRFP1, the putative ortholog of SDIR1, encodes a RING-H2 E3 ubiquitin ligase and responds to drought stress in an ABA-dependent manner in maize[J]. Gene, 495(2): 146-153. [26] Xiang D J, Man L L, Cao S, et al.2020. Ectopic expression of an oat SnRK2 gene, AsSnRK2D, enhances dehydration and salinity tolerance in tobacco by modulating the expression of stress-related genes[J]. Brazilian Journal of Botany, 43(3): 429-446. [27] Xu F Q, Xue H W.2019. The ubiquitin‐proteasome system in plant responses to environments[J]. Plant, Cell & Environment, 42(10): 2931-2944. [28] Yildiz H, Ercisli S, Hegedus A, et al.2014. Bioactive content and antioxidant characteristics of wild (Fragaria vesca L.) and cultivated strawberry (Fragaria×ananassa Duch.) fruits from Turkey[J]. Journal of Applied Botany and Food Quality, 87(1): 274-278. [29] Zhang H, Zhu J, Gong Z, et al.2022. Abiotic stress responses in plants[J]. Nature Reviews Genetics, 23(2): 104-119. [30] Zhang Y, Yang C, Li Y, et al.2007. SDIR1 is a RING finger E3 ligase that positively regulates stress-responsive abscisic acid signaling in Arabidopsis[J]. The Plant Cell, 19(6): 1912-1929. |
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