注射IAG重组质粒对克氏原螯虾IAG、mhc、gasp的表达及第二性征的影响

doi:10.3969/j.issn.1674-7968.2025.07.015

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Abstract The insulin-like androgenic gland hormone (IAG) gene plays an important role in sexual differentiation and growth in decapod crustaceans. To investigate the effects of recombinant plasmids containing IAG on the expression of endogenous genes (IAG) and growth-related genes myosin heavy chain(mhc) and growth arrest-specific protein (gasp) as well as secondary sexual characteristics in Procambarus clarkii. This study involved injecting different doses (0.5, 1.0 and 3.0 μg/g) of IAG recombinant plasmids into the muscle of healthy minor female P. clarkii weighing between 6.5~16.5 g. The weight, abdominal development, and expression of genes IAG, mhc, and gasp in the tissues of the experimental shrimp were monitored at 1, 7, 14, and 28 d, respectively. The results showed that compared with the control group, the copy number of plasmids in the experimental group was positively correlated with the concentration of injection. The copy number in each tissue showed a distribution pattern of muscle>gonads>appendages>hepatopancreas. The degree of plasmid digestion was the highest between 3 and 7 d, and at 28 d, the plasmid was almost completely digested. After 1 d of injection, the expression of mhc in muscles significantly increased at injection concentrations of 1.0 and 3.0 μg/g (P<0.05), and body weight gain was also significantly higher than that of the control group (P<0.05). Within 28 d after injection, female crayfish with an injection concentration of 3.0 μg/g had the fastest ovarian development, while the control group had the slowest development. No masculinization was observed in the abdomen of all female shrimps. Before the mass digestion of plasmids, the expression levels of IAG in the gonads and gasp in the muscles of the experimental group were lower than those in the control group, and their expression was inhibited. The results showed that the injection of IAG recombinant plasmid had certain effects on ovarian development and expression of IAG, mhc, gasp. This study provides reference for research on IAG function and gender control.

Key words： Procambarus clarkii Insulin-like androgenic gland hormone (IAG) Recombinant plasmid Myosin heavy chain (mhc) Growth arrest-specific protein (gasp)

Received: 04 August 2024

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Corresponding Authors: ^*liyanhe@mail.hzau.edu.cn

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	CHEN Yi-Jun
	XIE Ying-Lin
	ZHAO Tong
	YING Ke
	LI Yan-He

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CHEN Yi-Jun,XIE Ying-Lin,ZHAO Tong, et al. Effects of IAG Recombinant Plasmid Injection on the Expression of IAG, mhc, gasp and Secondary Sexual Characteristics of Procambarus clarkii[J]. 农业生物技术学报, 2025, 33(7): 1589-1597.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2025.07.015 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2025/V33/I7/1589

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