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Effects of Phytosterol Esters on Mice (Mus musculus) Skeletal Muscle Development |
FENG Xiao-Hua*, TAN Wei-Hao*, SUN Li-Juan, ZHAO Wei-Jie, CHEN Lyu-Shuang, XIE Kai-Lai, ZHU Shu-Qing, FENG Xia-Jie, SUN Zhong-Hua, WU Xin, WANG Li-Na** |
Guangdong Provincial Key Laboratory of Animal Nutrition Control/College of Animal Science, South China Agricultural University, Guangzhou 510642, China |
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Abstract Phytosterol esters (PE) are active substances formed by esterification of phytosterols with fatty acids. In order to investigate the effects of PE on skeletal muscle development in mice (Mus musculus), 3-week-old mice were randomly divided into control and experimental groups (i.e., the 0.3% PE group), and the effects of PE on muscle development were detected by feeding test, body composition, grip strength and weight lifting test, changes in myofibre area and type as well as myogenic factors were detected by immunofluorescence and qPCR, and finally, the interleukin content and its receptor downstream signaling pathway were analysed by ELISA and Western blot. The results showed that, 1) Dietary supplementation with 0.3% PE significantly increased the average daily intake of mice (P<0.05), and the gastrocnemius index was significantly up-regulated (P<0.05), while the subcutaneous fat index showed a down-regulation trend. 2) Dietary supplementation with 0.3% PE significantly improved the grip strength and weight lifting scores of mice (P<0.05), while there was no significant change in the running time and the distance of the run. 3) Dietary supplementation with 0.3% PE significantly increased the mean muscle fibre cross-sectional area of gastrocnemius and soleus muscles (P<0.05), significantly up-regulated myogenic factor 5 (Myf5) mRNA levels (P<0.05), and there was a tendency for up-regulation in the levels of myogenic differentiation (MyoD) and myogenin proteins as well. (4) Dietary supplementation of 0.3% PE significantly increased serum levels of interleukin-11 (IL-11)(P<0.05), Janus kinase 2 (JAK2) phosphorylation level had a tendency to increase, and signal transducers and activators of transcription 3 (STAT3) phosphorylation level was significantly increased (P<0.05). In conclusion, dietary addition of phytosterol esters could promote skeletal muscle development, increase muscle fibre diameter and enhance muscle function, which might be related to IL-11 and its downstream JAK2/STAT3 signaling pathway. This study provides a theoretical basis for phytosterol esters to promote muscle development in production.
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Received: 19 June 2024
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Corresponding Authors:
**wanglina@scau.edu.cn
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About author:: * These authors contributed equally to this work |
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[1] 程丽艳, 屠凌岚, 史红. 2015. 植物甾醇对慢性非细菌性前列腺炎症的干预作用及其机制研究[J]. 中国临床药理学与治疗学, 20(12): 1348-1353. (Cheng L Y, Tu L L, Shi H.2015. Study of the effects of Phytosterols in neuralgia in rats with chronic nonbacterial prostatitis and the mechanism[J]. Chinese Journal of Clinical Pharmacology and Therapeutics , 20(12): 1348-1353.) [2] 何昭友. 2012. 植物甾醇对肥育猪生长性能、血清指标及猪肉品的影响研究[D]. 硕士学位论文, 广西大学, 导师: 何若钢, 黄敏瑞, pp. 1-2. (He Z Y.2013. On influence of phytosterols on growth performance serum hormones and pork quality of fattening pigs[D]. Thesis for M. S., Guangxi University, Supervisor: He R G, Huang M R, PP. 1-2.) [3] 贾代汉, 周岩民, 王恬. 2007. 植物甾醇对肉鸡血清胆固醇、蛋白质水平和抗氧化酶活性的影响[J]. 中国粮油学报, (02): 88-93. (Effects of phytosterol on cholesterol and protein level and antioxidation enzyme activity in serum of broilers[J]. Journal of the Chinese Cereals and Oils Association, (02): 88-93.) [4] 马佩云, 孙超, 张忠品, 等. 2010. JAK2/STAT3信号通路对小鼠骨骼肌发育和能量代谢相关基因mRNA表达的影响[J]. 农业生物技术学报, 18(05): 951-955. (Ma P Y, Sun C, Zhang Z P, et al.Effects of JAK2/STAT3 signal pathway on mRNA expression of skeletal muscle development and energy metabolic related genes in mouse[J]. Journal of Agricultural Biotechnology, 18(05): 951-955.) [5] 明珠, 陈庆森, 张蕾, 等. 2017. 植物甾醇及添加植物甾醇乳制品降低心脑血管疾病风险的功能性评价(英文)[J]. 食品科学, 38(17): 174-183. (Ming Z, Cheng Q S, Zhang L, et al.2017. Evaluation of the efficacy of phytosterol and phytosterol-supplemented dairy products in reducing the risk of cardiovascular and cerebrovascular diseases[J]. Food Science, 38(17): 174-183.) [6] 吴先华, 何若钢, 覃小荣, 等. 植物甾醇对生长育肥猪生长性能、抗氧化能力及免疫功能的影响[J]. 中国饲料, 2013(16): 30-32. (Wu X H, He R G, Qin X R, et al. 2013. Effects of phytosterol on growth performance blood biochemical index and antioxidant index[J].China Feed, (16): 30-32.) [7] 章娜, 杨凯丽, 张沙, 等. 2023. 植物甾醇酯对产蛋末期蛋鸡产蛋性能、蛋品质、肝脏抗氧化能力及卵黄前体物质合成的影响[J]. 动物营养学报, 35(11): 7123-7137. (Zhang N, Yang K L, Zhang S, et al.2023. Effects of phytosterol ester on laying performance, egg quality, liver antioxidant capacity and yolk precursor synthesis of laying hens during late laying period[J]. Chinese Journal of Animal Nutrition, 35(11): 7123-7137.) [8] 卓锐文, 陈罡, 杨凯丽, 等. 2022. 饲粮中添加酯化植物甾醇对断奶仔猪生长性能、养分表观消化率、血清生化指标、回肠消化酶活性及肠道免疫指标的影响[J]. 动物营养学报, 34(07): 4237-4248. (Zhuo R W, Chen G, Yang K L, et al.2022. Effects of dietary phytosterol esters on growth performance, nutrient apparent digestibilities, serum biochemical indices, ileal digestive enzyme activities and intestinal immune indices of weaned piglets[J]. Chinese Journal of Animal Nutrition, 34(07): 4237-4248.) [9] Blanco-Vaca F, Cedo L, Julve J.2019. Phytosterols in cancer: From molecular mechanisms to preventive and therapeutic potentials[J]. Current Medicinal Chemistry, 26(37): 6735-6749. [10] Cioccoloni G, Soteriou C, Websdale A, et al.2022. Phytosterols and phytostanols and the hallmarks of cancer in model organisms: A systematic review and meta-analysis[J]. Critical Reviews in Food Science and Nutrition, 62(5): 1145-1165. [11] Hu Q, Zhuo Z, Fang S, et al.2017. Phytosterols improve immunity and exert anti-inflammatory activity in weaned piglets[J]. Journal of the Science of Food and Agriculture, 97(12): 4103-4109. [12] Jayaraman S, Devarajan N, Rajagopal P, et al.2021. Beta-sitosterol circumvents obesity induced inflammation and insulin resistance by down-regulating IKKbeta/NF-kappaB and JNK signaling pathway in adipocytes of type 2 diabetic rats[J]. Molecules, 26(7): 2101. [13] Liu J, Iqbal A, Raslawsky A, et al.2016. Influence of maternal hypercholesterolemia and phytosterol intervention during gestation and lactation on dyslipidemia and hepatic lipid metabolism in offspring of Syrian golden hamsters[J]. Molecular Nutrition and Food Research, 60(10): 2151-2160. [14] Luo X, Su P, Zhang W.2015. Advances in microalgae-derived phytosterols for functional food and pharmaceutical applications[J]. Marine Drugs, 13(7): 4231-4254. [15] Owen K L, Brockwell N K, Parker B S.2019. JAK-STAT signaling: A double-edged sword of immune regulation and cancer progression[J]. Cancers, 11(12): 2002. [16] Richardson P J, Smith D P, de Giorgio A, et al.2023. Janus kinase inhibitors are potential therapeutics for amyotrophic lateral sclerosis[J]. Translational Neurodegeneration, 12(1): 1-47. [17] Sousa-Victor P, Garcia-Prat L, Munoz-Canoves P.2022. Control of satellite cell function in muscle regeneration and its disruption in ageing[J]. Nature Review Molecular and Cellular Biology, 23(3): 204-226. [18] Ye W, Wu W, Lai J.2024. Effects of dietary phytosterols or phytosterol esters supplementation on growth performance, biochemical blood indices and intestinal flora of C57BL/6 mice[J]. PLOS ONE, 19(5): 1-29. [19] Zammit P S.2017. Function of the myogenic regulatory factors Myf5, MyoD, Myogenin and MRF4 in skeletal muscle, satellite cells and regenerative myogenesis[J]. Seminars in Cell and Developmental Biology, 72: 19-32. [20] Zhao W, Su H, Wang L, et al.2019. Effects of maternal dietary supplementation of phytosterol esters during gestation on muscle development of offspring in mice[J]. Biochemical and Biophysical Research Communications, 520(2): 479-485. |
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