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| Identification of the Prunus mume GATA Gene Family and Analysis of the Role of PmGATA12 in Cold Tolerance Regulation |
| LIU Zhao-Liang1, ZHANG Jun2, JIN Xiao-Jun2, JIN Song-Heng3, DONG Bin4* |
1 Beijing Orient Landscape and Environment Co., Ltd, Beijing 100020, China;
2 Tongchuang Engineering Design Co. Ltd, Shaoxing 312000, China;
3 Jiyang college, Zhejiang A&F University, Zhuji 311800, China;
4 School of Landscape Architecture, Zhejiang A&F University, Hangzhou 311300, China |
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Abstract Prunus mume, a traditional famous flower in China, holds significant ornamental value. GATA transcription factors play a crucial role in the regulation of plant growth and development as well as in the response to abiotic stress. In this study, a genome-wide identification of the GATA gene family in P. mume was conducted, and their expression patterns following cold treatment were systematically analyzed, with the functional characterization of PmGATA12 being comprehensively investigated. The results indicated that a total of 20 PmGATA genes were identified through whole-genome analysis, distribute across 7 chromosomes. Phylogenetic analysis revealed that PmGATA proteins could be divided into 3 subfamilies. Members of the same subfamily have similar conserved motifs and gene structures. Furthermore, by integrating transcriptome and qRT-PCR expression analysis, 4 differentially expressed PmGATA genes (PmGATA6, PmGATA11, PmGATA12, and PmGATA14) were identified. The genetic transformation of Arabidopsis thaliana with PmGATA12 significantly enhanced the cold tolerance of the transgenic lines. This study provides valuable gene resources for the cold-resistance breeding of P. mume and offers new insights into the molecular mechanisms underlying its cold tolerance.
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Received: 11 October 2024
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Corresponding Authors:
* dongbin@zafu.edu.cn
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