Cloning and Expression Analysis of TaPM19-B1 Gene in Wheat (Triticum aestivum)
HUANG Li-Dan1,2, WANG De-Zhou2, WANG Xiao-Yan1*, TANG Yi-Miao2*
1 College of Agriculture, Yangtze University, Jingzhou 434200, China; 2 Institute of Hybrid Wheat, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China
Abstract In recent years, numerous genes associated with wheat (Triticum aestivum) grain size and dormancy have been identified through studies on the genetic basis controlling these traits. Based on abscisic acid (ABA) induced wheat plasma membrane 19 (AWPM19) protein family member TaPM19-B1 (TraesCS5B02G566400), this study cloned the full-length TaPM19-B1 gene (543 bp) from the cDNA of the 'Chinese Spring' wheat variety, which encoded a protein of 180 amino acids. Subcellular localization prediction of wheat protoplasts indicated that the TaPM19-B1 protein was localized to the cell membrane. Analysis of the cis-acting elements in the TaPM19-B1 promoter region revealed that the presence of multiple regulatory elements associated with seed development, stress response, and phytohormone signaling. RNA-Seq transcriptome data of wheat and qPCR validation demonstrated that TaPM19-B1 was scarcely expressed in the wheat root, stem, leaf, and spike, but was significantly expressed during the later stages of grain development, with the highest expression levels observed at 10 and 25 days after anthesis (DAA). Furthermore, the expression of TaPM19-B1 was significantly upregulated under drought, salt, heat, and ABA stress conditions. Additionally, an analysis of TaPM19-B1 gene expression at different stages of grain development in 2 large-grain varieties (1000-grain weight>50 g) and 2 small-grain varieties (1000-grain weight<21 g) revealed that TaPM19-B1 expression was significantly higher in the large-grain varieties (P<0.05). This study provides theoretical insights into the molecular regulatory mechanisms of TaPM19-B1 in wheat growth, development, and response to environmental stresses.
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