Identification and Expression Analysis of Trehalose-6- Phosphate Synthase Family in Casuarina equisetifolia
ZHANG Gui-Hua1,*, YUE Chun-Lei1, LI He-Peng1, ZHANG Xiao-Mian1, YU Zhen-Ming2
1 Zhejiang Academy of Forestry, Hangzhou 310023, China; 2 School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 311402, China
Abstract Trehalose-6-phosphate synthase (TPS) is the crucial rate-limiting enzyme for the trehalose biosynthesis, and plays a significant role in the stress responsiveness. To investigate the function of TPS regarding growth and development, stress response in Casuarina equisetifolia, physicochemical features, chromosomal localization, phylogenetic tree, conserved motifs, gene structure, cis-regulatory elements and collinearity were analyzed in this study. Their expression patterns in different tissues, and exposed to drought, salt stress and abscisic acid (ABA) treatment were determined. Results indicated that 7 CeTPS genes were randomly distributed in 4 chromosomes, encoding 826~998 amino acids that were hydrophilic. The CeTPS proteins were divided into 2 subfamilies through phylogenetic analysis, showing relatively close to dicotyledonous plants, and harbored gene duplications. The promoter regions of CeTPS genes were rich in cis-acting elements involved in hormonal responsiveness, growth and development, and stress responsiveness. CeTPS genes showed tissue-specific expression, and were differentially expressed under drought, salt stress, and ABA treatment. Especially, CeTPS4 might play an essential role in stress responsiveness. The results lay a foundation for in-depth study of the CeTPS genes associated with abiotic stress responsiveness.
ZHANG Gui-Hua,YUE Chun-Lei,LI He-Peng, et al. Identification and Expression Analysis of Trehalose-6- Phosphate Synthase Family in Casuarina equisetifolia[J]. 农业生物技术学报, 2025, 33(3): 581-593.
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