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| Identification and Expression Analysis of COBL Gene Family in Diploid Species Arachis duranensis, Arachis ipaensis and Arachis hypogaea |
| ZHANG Wan-Nian, LI Yu-Fa, WANG Wei, LIU Hong-Xin, YANG Xiang-Yu, LI Wei-Tang, NIU Hai-Long* |
| Peanut Research Institute, Jilin Academy of Agricultural Sciences, Changchun 136100, China |
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Abstract The COBL (COBRA-like) gene family constitutes a significant gene family, which is involved in the regulation of cell wall amplification. To investigate the function of COBL genes in peanut (Arachis hypogaea), diploid species peanuts Arachis duranensis and Arachis ipaensis , this study mined and identified the peanut COBL gene family and analyzed them for their physicochemical properties, conserved structures, phylogenetic trees and tissue expression patterns. The results showed that there were a total of 51 COBL gene family members in cultivated peanut, diploid peanuts Arachis duranensis and Arachis ipaensis. The peanut COBL gene family members were analogous to those of Arabidopsis thaliana and soybean (Glycine max), which were divided into 2 subfamilies. Each subfamily contained genes that were homologous to those in A. thaliana and soybean. These homologous genes exhibited a high degree of sequence consistency, which indicated the conservativeness of the COBL family genes. The COBL family was shown to to play a pivotal role in the growth and development of the peanut at various stages of its lifecycle. This finding suggested that COBL genes played important regulatory roles in the development of multiple tissues. In the context of salt and drought stress, the expression levels of certain genes in the roots were found to be elevated, suggesting that the activation of these genes in the roots played a crucial role in the perception and response to stress signals when plants were subjected to adverse environmental conditions. This study provides a reference for subsequent functional studies of peanut COBL genes.
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Received: 14 February 2025
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Corresponding Authors:
* Corresponding author, jlccnhld@126.com
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