1 College of Life Science and Biotechnology, Gansu Agricultural University, Lanzhou 730070, China; 2 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; 3 Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation, Lanzhou 730070, China
Abstract The development of mammalian placenta is crucial for regulation of intrauterine environment and pregnant maintenance. Dysplasia or abnormality placenta could cause a variety of pregnant complications. Stabilin-1 (STAB1) is involved in regulating the development of several organs in animals, but its function and regulatory mechanisms are not clearly understood in yak (Bos grunniens) placenta. This study aimed to investigate the distribution, expression pattern and potential function of STAB1 in yak placenta during early and middle pregnancy. Yak placental tissues were collected at early and middle pregnancy. The distribution and expression pattern of STAB1 in yak placental tissues during early and middle pregnancy were observed using hematoxylin-eosin (HE) staining, immunohistochemistry (IHC) staining, immunofluorescence (IF) staining, qRT-PCR and Western blot. Meanwhile, the interacting proteins and potential functions of STAB1 were predicted based on data-independent acquisition (DIA) proteomics data. The results showed that STAB1 protein was mainly localized in the cytoplasm of yak placenta trophoblast giant cells and uninucleate trophoblast cells. Compared to early pregnancy placenta, the expression of STAB1 gene and its protein levels were significantly down-regulated in yak middle pregnancy (P<0.05). Bioinformatics analysis revealed that STAB1 might regulate cell adhesion, calcium binding and plasma membrane composition and other pathways involved in the dynamic development of yak's placenta during the early and middle pregnancy. In conclusion, STAB1 might be involved in placental development and pregnancy maintenance in early and middle pregnancy through regulating the function of placental trophoblast cells in yaks. This study provides a reference for further investigation of the function and mechanism of STAB1 in yak placental growth and development.
WEI Qian-Qian,ZHANG Quan-Wei,ZHANG Bo-Hao, et al. Expression, Distribution and Function Analysis of STAB1 in the Placenta of Yak (Bos grunniens) Early and Middle Pregnancy Phase[J]. 农业生物技术学报, 2024, 32(4): 785-794.
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