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| Identification of the Pathogen Causing Mosaic Leaves in Seed Zucchini (Cucurbita pepo) in Xinjiang |
| HAO Ting-Li, CHEN Da-Qun, ZHANG Yan, DU Zhen-Zhu, WU Qin, LIU Sheng-Xue* |
| Analysis and Testing Center, Shihezi University, Shihezi 832003, China |
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Abstract Seed Zucchini (Cucurbita pepo), a major economic crop in Xinjiang, suffers severe yield and quality losses due to viral infections, and identifying its pathogens is essential for further control measures. In this study, leaf samples displaying mosaic symptoms were collected from Seed Zucchini in Shihezi, Xinjiang. Under an electron microscope, flexuous Potyvirus particles measuring approximately 750 × 11 nm were observed. The double-stranded RNA (dsRNA) of the virus was extracted, RT-PCR was performed on the virus with random primers and diffuse bands XHL2-1, XHL2-2, and XHL56 were obtained. BLAST searches in the NCBI database revealed that XHL2-1 and XHL56 had sequence similarities of 92.22%~98.69% and 96.21%~99.64%, respectively, with the corresponding regions of Zucchini yellow mosaic virus (ZYMV); XHL2-2 showed a sequence similarity of 89.38%~98.45% with the corresponding region of Watermelon mosaic virus (WMV). These findings indicated that the mosaic disease in Seed Zucchini might be caused by infections of both ZYMV and WMV. To further investigate, specific primers for ZYMV and WMV were designed and synthesized. RT-PCR was conducted on 15 samples, and ZYMV-specific amplification fragments from samples SH2, SH12, and SH13 were sequenced. The sequence similarity among these fragments was over 99.29%, with a similarity of 96.09%~99.56% to ZYMV sequences recorded in GenBank. The WMV-specific amplification fragments from samples SH1, SH9, and SH11 had sequence similarities exceeding 94.30% among themselves, and 94.74%~100% similarity with WMV sequences in GenBank. These results further confirmed that the mosaic disease in Seed Zucchini in Xinjiang was caused by co-infection of ZYMV and WMV. Phylogenetic analysis revealed that the ZYMV infecting Seed Zucchini in Xinjiang clusters with isolates from Eastern Europe and India, while the WMV was closely related to isolates from China, France, and other regions. This study identifies the primary pathogens of mosaic virus disease in Xinjiang's Seed Zucchini as a co-infection by ZYMV and WMV, providing a theoretical basis for further control measures against the disease.
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Received: 10 November 2023
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Corresponding Authors:
*liushengxue-1@163.com
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