Genome Wide Identification and Expression Analysis of the HD-Zip Gene Family in Pinellia ternata
LIU Meng-Meng1, LIU Xiao1, YOU Qian1, BO Chen1, ZHU Yan-Fang1, DUAN Yong-Bo1, XUE Jian-Ping1, WANG De-Xin2,*, XUE Tao1,*
1 College of Life Sciences/Anhui Provincial Engineering Laboratory for Efficient Utilization of Featured Resource Plants, Huaibei Key Laboratory of Efficient Cultivation and Utilization of Resource Plants; Huaibei Normal University, Huaibei 235000, China; 2 College of Agriculture and Engineering, Heze University, Heze 274009, China
Abstract The sprout tumble (ST) of Pinellia ternata caused by environmental sress severely limits its industrial development, and the homeodomain-leucine zipper (HD-Zip) family genes are widely involved in the regulation of plant stress response. To explore the HD-Zip genes involved in the regulation of ST in P. ternata, the HD-Zip family genes of P. ternata were identified and analyzed at the genome level in this study. It was found that 17 HD-Zip family genes were identified in P. ternata, among them, 15 of which were attached to 9 chromosomes. Evolutionary analysis showed that the identified 17 HD-Zip family members included 9 HD-Zip Ⅰ subfamily members and 8 HD-Zip Ⅱ subfamily members, all of which had highly conserved HD domains (WFQNRR) in their protein sequences. Subcellular localization prediction showed that all 17 members of the HD-Zip family were located in the nucleus, and collinearity analysis showed that 8 PtHDZ genes formed 4 pairs of homologous gene pairs, and 1 genes had a collinearity relationship with rice (Oryza sativa). The elements of hormones response, stress response, and transcription factor binding sites were contained in the promoters of PtHDZ genes via the prediction analysis of cis acting elements. Organizational expression pattern analysis revealed that the PtHDZ family genes showed differential expression in different tissues, most of which were highly expressed in roots and tubers. And the expression of 14 PtHDZs were found to be induced while 2 PtHDZs were inhibited by high temperature. This study provides genetic resources for analyzing the mechanism of ST and molecular breeding in P. ternata.
LIU Meng-Meng,LIU Xiao,YOU Qian, et al. Genome Wide Identification and Expression Analysis of the HD-Zip Gene Family in Pinellia ternata[J]. 农业生物技术学报, 2024, 32(11): 2540-2551.
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