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| Exploring the Anti-inflammatory Effects of Peimine and Peiminine on Macrophage Raw264.7 Based on LPS Inflammation Model |
| JIN Ze-Lan1,*, DONG Yun-Zhe1,2,*, LI Qing1, DONG Li-Li1, GUO Hua-Ru3, YAN Can-Long1, WANG Zhong-Hua1,** |
1 College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China;
2 School of Life Sciences, Ningxia University, Yinchuan 750000, China;
3 Ningbo Agricultural Technology Extension Station, Ningbo 315100, China |
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Abstract Peimine and Peiminine are representative alkaloid medicinal component in the lily family herb Fritillaria thunbergii. They play an important role in the anti-inflammatory effect of F. thunbergii. In order to further investigate the anti-inflammatory effects of these 2 types of alkaloids at the cellular level, this study used lipopolysaccharide (LPS) induced Raw264.7 cells as an inflammatory model, Griess, ELISA, Western blot and qRT-PCR were used to analyze the effects of peimine and peiminine on LPS-induced secretion of pro-inflammatory factors nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-1β, nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in Raw264.7 cells, the results showed that both peimine and peiminine could inhibit the differentiation of Raw264.7 cells and reduce the pseudopodia produced by LPS stimulation, NO, TNF-α, IL-6 and IL-1β in Raw264.7 cell supernatant of medium and high dose groups of peimine and peiminine significantly decreased (P<0.05, P<0.01). Peimine and peiminine inhibited the expression of iNOS and COX-2 protein in Raw264.7 cells, and the inhibition of peiminine on iNOS protein was higher than that of peiminine on COX-2 protein, the inhibitory effect of peiminine a was higher than that of peiminine. The expression of IL-6 and IL-1β in Raw264.7 cells was significantly decreased by both peimine and peiminine. In conclusion, both peimine and peiminine have good anti-inflammatory effect, which provides some data support for the treatment of inflammation by alkaloids.
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Received: 06 September 2023
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Corresponding Authors:
** wang1972@zwu.edu.cn
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| About author:: * These authors contributed equally to this work |
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