|
|
|
| Preliminary Study on ZmMYC7 Transcription Factor Regulating of Disease Resistance Related Functions in Maize (Zea mays) |
| WANG Yong-Gui1,*, LI Wei1,*, CUI Ting-Ru2, ZHANG Fu-Yuan1, YANG Wen-Hao1, XU Dang-Fei3, XING Ji-Hong1, CAO Hong-Zhe1,**, DONG Jin-Gao1,** |
1 State Key Laboratory of North China Crop Improvement and Regulation/Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding 071000, China;
2 Baoding Meteorological Service, Baoding 071000, China;
3 Zhangjiakou Forestry Station, Zhangjiakou 075000, China |
|
|
|
|
Abstract Maize (Zea mays) is one of the most important food crops in the world. Maize diseases caused by pathogens directly affect maize production and lead to huge economic losses. Transcription factor MYC2 plays an important role in the field of plant disease resistance. ZmMYC7 (Zm00001d030028), ortholog of Arabidopsis thaliana AtMYC2, was previously obtained in the previous work. In order to explore the function of transcription factor ZmMYC7 in regulating maize disease resistance, the sensitivity of ZmMYC7 mutant to 4 maize fungal diseases was detected, and the effect of ZmMYC7 mutation on the expression level of pathogenesis-related protein gene ZmPRs was detected by qRT-PCR. The results showed that ZmMYC7 had the highest similarity (90%) with Sorghum bicolor SbMYC2. ZmMYC7 was highly expressed in leaves and anthers of maize. The expression of ZmMYC7 was up-regulated by UV irradiation and Fusarium graminearum infection. In ZmMYC7 mutants, the sensitivity of maize to leaf and stem diseases was significantly increased, and the expression level of disease resistance related gene ZmPRs was significantly down-regulated (P<0.01). The above results showed that ZmMYC7 played an important role in maize resistance to pathogen disease. The present study provides basic materials for further analysis of the molecular mechanism of ZmMYC7 regulating maize disease resistance.
|
|
Received: 02 February 2023
|
|
|
|
Corresponding Authors:
** Corresponding authors, caohongzhe1986@hotmail.com; dongjingao@126.com
|
| About author:: * These authors contributed equally to this work |
|
|
|
[1] 刘庆霞, 李梦莎, 国静. 2012. 茉莉酸生物合成的调控及其信号通路[J]. 植物生理学报, 48(9): 837-844.
(Liu Q X, Li M S, Guo J, 2012. Regulation of jasmonic acid biosynthesis and jasmonic acid signaling pathway[J]. Plant Physiology Journal, 48(9): 837-844.)
[2] 王振营, 王晓鸣. 2019. 我国玉米病虫害发生现状、趋势与防控对策[J]. 植物保护, 45(1): 1-11.
(Wang Z Y, Wang X M.2019. Current status and management strategies for corn pests and diseases in China[J]. Plant Protection, 45(1): 1-11.)
[3] Anderson J P, Badruzsaufari E, Schenk P M, et al.2004. Antagonistic interaction between abscisic acid and jasmonate-ethylene signaling pathways modulates defense gene expression and disease resistance in Arabidopsis[J]. The Plant Cell, 16(12): 3460-3479.
[4] Anisimova O K, Shchennikova A V, Kochieva E Z, et al.2021. Pathogenesis-related genes of PR1, PR2, PR4, and PR5 families are involved in the response to infection in garlic (Allium sativum L.)[J]. International Journal of Molecular Sciences, 22(13): 6688.
[5] Cao J, Li M, Chen J, et al.2016. Effects of MeJA on Arabidopsis metabolome under endogenous JA deficiency[J]. Scientific Reports, 6: 37674.
[6] Chadha P, Das R H.2006. A pathogenesis related protein, AhPR10 from peanut: An insight of its mode of antifungal activity[J]. Planta, 225(1): 213-222.
[7] Cheng Z, Sun L, Qi T, et al.2011. The bHLH transcription factor MYC3 interacts with the Jasmonate ZIM-domain proteins to mediate jasmonate response in Arabidopsis[J]. Molecular Plant, 4(2): 279-288.
[8] Chico J M, Lechner E, Fernandez-Barbero G, et al.2020. CUL3BPM E3 ubiquitin ligases regulate MYC2, MYC3, and MYC4 stability and JA responses[J]. Proceedings of the National Academy of Sciences of the USA, 117(11): 6205-6215.
[9] Dombrecht B, Xue G P, Sprague S J, et al.2007. MYC2 differentially modulates diverse jasmonate-dependent functions in Arabidopsis[J]. The Plant Cell, 19(7): 2225-2245.
[10] Du M, Zhao J, Tzeng D, et al.2017. MYC2 orchestrates a hierarchical transcriptional cascade that regulates jasmonate-mediated plant immunity in tomato[J]. The Plant Cell, 29(8): 1883-1906.
[11] Fernández-Calvo P, Chini A, Fernández-Barbero G, et al.2011. The Arabidopsis bHLH transcription factors MYC3 and MYC4 are targets of JAZ repressors and act additively with MYC2 in the activation of jasmonate responses[J]. The Plant Cell, 23(2): 701-715.
[12] Fu J, Liu L, Liu Q, et al.2020. ZmMYC2 exhibits diverse functions and enhances JA signaling in transgenic Arabidopsis[J]. Plant Cell Reports, 39(2): 273-288.
[13] Kazan K, Manners J M.2013. MYC2: The master in action[J]. Molecular Plant, 6(3): 686-703.
[14] Lorenzo O, Chico J M, Sánchez-Serrano J J, et al.2004. JASMONATE-INSENSITIVE1 encodes a MYC transcription factor essential to discriminate between different jasmonate-regulated defense responses in Arabidopsis[J]. The Plant Cell, 16(7): 1938-1950.
[15] Lotan T, Ori N, Fluhr R.1989. Pathogenesis-related proteins are developmentally regulated in tobacco flowers[J]. The Plant Cell, 1(9): 881-887.
[16] Pré M, Atallah M, Champion A, et al.2008. The AP2/ERF domain transcription factor ORA59 integrates jasmonic acid and ethylene signals in plant defense[J]. Plant Physiology, 147(3): 1347-1357.
[17] Pungartnik C, da Silva A C, de Melo S A, et al.2009. High-affinity copper transport and Snq2 export permease of Saccharomyces cerevisiae modulate cytotoxicity of PR-10 from Theobroma cacao[J]. Molecular Plant-Microbe Interactions, 22(1): 39-51.
[18] Santana M F, Silva J C F, Mizubuti E S G, et al.2014. Characterization and potential evolutionary impact of transposable elements in the genome of Cochliobolus heterostrophus[J]. BMC Genomics, 15: 536.
[19] Sun A, Yu B, Zhang Q, et al.2020. MYC2-activated TRICHOME BIREFRINGENCE-LIKE37 acetylates cell walls and enhances herbivore resistance[J]. Plant Physiology, 184(2): 1083-1096.
[20] Tornero P, Gadea J, Conejero V, et al.1997. Two PR-1 genes from tomato are differentially regulated and reveal a novel mode of expression for a pathogenesis-related gene during the hypersensitive response and development[J]. Molecular Plant-Microbe Interactions, 10(5): 624-634.
[21] Valenzuela-Riffo F, Zúñiga P E, Morales-Quintana L, et al.2020. Priming of defense systems and upregulation of MYC2 and JAZ1 genes after Botrytis cinerea inoculation in methyl jasmonate-treated strawberry fruits[J]. Plants (Basel, Switzerland), 9(4): 447.
[22] Wanzel M, Herold S, Eilers M.2003. Transcriptional repression by Myc[J]. Trends in Cell Biology, 13(3): 146-150.
[23] Xie Y, Chen Z, Brown R L, et al.2010. Expression and functional characterization of two pathogenesis-related protein 10 genes from Zea mays[J]. Journal of Plant Physiology, 167(2): 121-130.
[24] Zhang D, Wang F, Zhao J, et al.2019. Virulence, molecular diversity, and mating type of Curvularia lunata in China[J]. Plant Disease, 103(7): 1728-1737.
[25] Zhang J, Wu Y, Ho H, et al.2014. BZcon1, a SANT/Myb-type gene involved in the conidiation of Cochliobolus carbonum[J]. G3 (Bethesda, Md.), 4(8): 1445-1453.
[26] Zribi I, Ghorbel M, Brini F.2021. Pathogenesis related proteins (PRs): From cellular mechanisms to plant defense[J]. Current Protein & Peptide Science, 22(5): 396-412. |
|
|
|
