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| Study on TcGRF3 in Regulating Salt Tolerance of Tamarix chinensis |
| LYU Meng-Hua, CHEN Qian-Qian, TIAN You-Zi, XIAO Dan-Dan, WANG Yuan-Yuan*, ZHANG Guo-Jun* |
| College of Horticulture Science and Technology, Hebei Normal University of Science and Technology/Hebei Key Laboratory of Horticultural Germplasm Excavation Innovative Utilization/Hebei Higher Institute Application Technology Research and Development Center of Horticultural Plant Biological Breeding, Qinhuangdao 066600, China |
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Abstract The 14-3-3 protein family, constitutes a class of highly conserved regulatory proteins in plants, also known as G?box regulatory factors (GRFs), are extensively involved in various physiological processes such as cellular metabolism, signal transduction, and responses to biotic and abiotic stresses. Tamarix chinensis is a typical recretohalophyte, but the function and regulatory mechanism of its 14-3-3 family genes in response to salt stress remains unclear. To investigate the characteristics of the 14-3-3 gene family in T. chinensis and their roles in salt tolerance, the study cloned, identified, and functionally analyzed 14-3-3 family genes. The results showed that 5 14-3-3 genes, named TcGRF1 to TcGRF5, respectively, were successfully cloned, and all TcGRF proteins contained the typical conserved 14-3-3 domain. qPCR analysis revealed that the expression levels of all 5 TcGRFs genes were up-regulated to varying degrees under 0.2 and 0.4 mol/L NaCl stress, with TcGRF3 showing the most obvious difference. To further clarify the function of TcGRF3 under salt stress, transient transformation technology was used to overexpress and suppress the expression of TcGRF3 in Tamarix chinensis, respectively. Overexpression of TcGRF3 significantly increased chlorophyll content, the activities of superoxide dismutase (SOD) and peroxidase (POD)(P<0.05), and proline accumulation, while decreasing relative electrical conductivity and malondialdehyde (MDA) content (P<0.05). In contrast, TcGRF3-silenced plants showed the opposite trends. Comprehensive analysis indicated that TcGRF3 positively regulated salt tolerance in T. chinensis by enhancing reactive oxygen species (ROS) scavenging capacity, thereby mitigating membrane lipid peroxidation damage. This study provides a theoretical foundation for further elucidating the functional mechanisms of the 14-3-3 protein family in the salt stress response of T. chinensis.
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Received: 15 December 2025
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| Fund:河北省中央引导地方科技发展资金(254Z6301G); 河北省现代农业产业技术体系花卉产业创新团队建设专项资金(HBCT2024200202); 河北省重点研发计划(21326301D); 河北科技师范学院博士研究启动基金(2024YB012) |
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Corresponding Authors:
*hljwangyuanyuan@nefu.edu.cn; zhangguojun-8@163.com
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[1] 王鹏洋, 赵欣雨, 李博洋, 等. 2025. 水分和氮磷添加对水曲柳幼苗生长及光合作用的影响[J]. 森林工程, 41(6): 1135-1144.
(Wang P Y, Zhao X Y, Li B Y, et al.2025. Effects of water and nitrogen-phosphorus additions on the growth and photosynthesis of Fraxinus mandshurica seedlings[J]. Forest Engineering, 41(6): 1135-1144.)
[2] 王晓东, 赵彩鸿, 王瑞瑞, 等. 2024. 聚乙二醇对盐碱胁迫下花楸胚胎萌发及幼苗生理指标的影响[J]. 森林工程, 40(4): 19-28.
(Wang X D, Zhao C H, Wang R R, et al.2024. Effects of polyethylene glycol on embryo germination and physiological indicators of Sorbus pohuashanensis embryos under saline alkali stress[J]. Forest Engineering, 40(4): 19-28.)
[3] 朱北艳, 李俊奇, 樊佳鑫, 等. 2026. 南方碱蓬MYB基因家族鉴定及关键耐盐基因功能验证[J]. 农业生物技术学报, 34(02): 306-322.
(Zhu B Y, Li J Q, Fan J X, et al.2026. Identification of MYB gene family in Suaeda australis and functional verification of key salt-tolerant genes[J]. Journal of Agricultural Biotechnology, 34(02): 306-322.)
[4] Aitken A.2002. Functional specificity in 14-3-3 isoform interactions through dimer formation and phosphorylation, chromosome location of mammalian isoforms and variants[J]. Plant Molecular Biology, 50(6): 993-1010.
[5] Amako K, Chen G X, Asada K.1994. Separate assays specific for ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolic isozymes of ascorbate peroxidase in plants[J]. Plant and Cell Physiology, 35: 497-504.
[6] Anna J W, Lenka S, Ljudmilla T, et al.2021. Arabidopsis bZIP18 and bZIP52 accumulate in nuclei following heat stress where they regulate the expression of a similar set of genes[J]. International Journal of Molecular Sciences, 22(2): 530.
[7] Cai J S, Cai W W, Huang X Y, et al.2021. Ca14-3-3 interacts with CaWRKY58 to positively modulate pepper response to low phosphorus starvation[J]. Frontiers in Plant Science, 11: 607878.
[8] Chen S T, Qiu G L.2022. 14-3-3 gene of Zostera japonica ZjGRF1 participates in gibberellin signaling pathway[J]. Molecular Biology Reports, 49(6): 4795-4803.
[9] De Vetten N C, Ferl R J.1994. Two genes encoding GF14 (14-3-3) proteins in Zea mays (structure, expression, and potential regulation by the G-box-binding complex)[J]. Plant Physiology, 106(4): 1593-1604.
[10] Han Y Y, Lou X, Zhang W R, et al.2022. Arbuscular mycorrhizal fungi enhanced drought resistance of Populus cathayana by regulating the 14-3-3 family protein genes[J]. Microbiology Spectrum, 10(3): e0245621.
[11] He F Y, Duan S G, Jian Y Q, et al.2022. Genome-wide identification and gene expression analysis of the 14-3-3 gene family in potato (Solanum tuberosum L.)[J]. BMC Genomics, 23(1): 811.
[12] Huang Y, Wang W S, Yu H, et al.2022. The role of 14-3-3 proteins in plant growth and response to abiotic stress[J]. Plant Cell Reports, 41(4): 833-852.
[13] Jia C P, Guo B, Wang B K, et al.2022. Genome-wide identification and expression analysis of the 14-3-3 (TFT) gene family in tomato, and the role of SlTFT4 in salt stress[J]. Plants, 11(24): 3491.
[14] Kramer G F, Norman H A, Krizek D T, et al.1991. Influence of UV-B radiation on polyamines, lipid peroxidation and membrane lipids in cucumber[J]. Phytochemistry, 30: 2101-2108.
[15] Liu J N, Fang H C, Liang Q, et al.2022. Genomic analyses provide insights into the evolution and salinity adaptation of halophyte Tamarix chinensis[J]. Giga Science, 12: 1-17.
[16] Ma Y M, Wu Z Y, Dong J F, et al.2023. The 14-3-3 protein OsGF14f interacts with OsbZIP23 and enhances its activity to confer osmotic stress tolerance in rice[J]. The Plant Cell, 35(11): 4173-4189.
[17] Moore B W, Perez V J.1967. Specific acidic proteins of the nervous system[J]. Physiological and Biochemical Aspects of Nervous Integration, 34: 343-359.
[18] Pan R, Ren W J, Liu S S, et al.2023. Ectopic over-expression of HaFT-1, a 14-3-3 protein from Haloxylon ammodendron, enhances acquired thermotolerance in transgenic Arabidopsis[J]. Plant Molecular Biology, 112(4-5): 261-277.
[19] Ren J, Wang H, Zhao M, et al.2025. MdGRF22, a 14-3-3 family gene in apple, negatively regulates drought tolerance via modulation of antioxidant activity and interaction with MdSK[J]. Plants, 14(13): 1968.
[20] Ren Y R, Yang Y Y, Zhang R, et al.2019. MdGRF11, an apple 14-3-3 protein, acts as a positive regulator of drought and salt tolerance[J]. Plant Science, 288:110219.
[21] Seung J C, Hyun J P, Seon J K, et al.2021. Early detection of plant stress using the internal electrical conductivity of Capsicum annuum in response to temperature and salinity stress[J]. Plant Growth Regulation, 95(3): 371-380.
[22] Song P Y, Yang Z D, Guo C, et al.2023. 14-3-3 proteins regulate photomorphogenesis by facilitating light-induced degradation of PIF3[J]. New Phytologist, 237(1): 140-159.
[23] Šribar J, Sherman N E, Prijatelj P, et al.2003. The neurotoxic phospholipase A2 associates, through a non-phosphorylated binding motif, with 14-3-3 protein γ and ε isoforms[J]. Biochemical and Biophysical Research Communications, 302(4): 691-696.
[24] Sun J, Zhang G C, Cui Z B, et al.2022a. Regain flood adaptation in rice through a 14-3-3 protein OsGF14h[J]. Nature Communications, 13(1): 5664.
[25] Sun Q F, Xi Y, Lu P P, et al.2022b. Genome-wide analysis of the G-box regulating factors protein family reveals its roles in response to Sclerotinia sclerotiorum infection in rapeseed (Brassica napus L.)[J]. Frontiers in Plant Science,13: 986635.
[26] Sun X, Luo X, Sun M, et al.2014. A Glycine soja 14-3-3 protein GsGF14o participates in stomatal and root hair development and drought tolerance in Arabidopsis thaliana[J]. Plant & Cell Physiology, 55: 99-118.
[27] Visconti S, D'Ambrosio C, Fiorillo A, et al.2019. Overexpression of 14-3-3 proteins enhances cold tolerance and increases levels of stress responsive proteins of Arabidopsis plants[J]. Plant Science, 289: 110215.
[28] Wang Q, Peng W Y, Rong J B, et al.2023. Molecular analysis of the 14-3-3 genes in Panax ginseng and their responses to heat stress[J]. PeerJ, 11:e15331.
[29] Wang Y Y, Liu Z Y, Wang P L, et al.2020. A 2-Cys peroxiredoxin gene from Tamarix hispida improved salt stress tolerance in plants[J]. BMC Plant Biology, 20(1): 360.
[30] Xia L M, He X H, Hu W L, et al.2023. Overexpression of the mango MiGF6A and MiGF6B genes promotes early flowering in transgenic Arabidopsis plants[J]. Scientia Horticulturae, 318: 112074.
[31] Yan J, He C, Wang J, et al.2004. Overexpression of the Arabidopsis 14-3-3 protein GF14 lambda in cotton leads to a 'stay-green' phenotype and improves stress tolerance under moderate drought conditions[J]. Plant & Cell Physiology, 45: 1007-1014.
[32] Zhang G, Wei B.2023. Characterization of 14-3-3 gene family and their expression patterns under abiotic and biotic stresses in melon (Cucumis melo L.)[J]. Horticulture, Environment, and Biotechnology, 64(6): 1039-1054.
[33] Zhang Z B, Wang X K, Wang S, et al.2022. Expansion and diversification of the 14-3-3 gene family in Camellia sinensis[J]. Journal of Molecular Evolution, 90(3-4): 296-306.
[34] Zhou H, Lin H, Chen S, et al.2014. Inhibition of the Arabidopsis salt overly sensitive pathway by 14-3-3 proteins[J]. The Plant Cell, 26: 1166-1182.
[35] Zhu R, Cao B, Sun M, et al.2023b. Genome-wide identification and evolution of the GRF gene family and functional characterization of PbGRF18 in pear[J]. International Journal of Molecular Sciences, 24(19): 14690.
[36] Zhu Y Q, Kuang W, Leng J, et al.2023a. The apple 14-3-3 gene MdGRF6 negatively regulates salt tolerance[J]. Frontiers in Plant Science, 14: 1161539. |
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