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| Polymorphism Identification of the ACSL6 Gene and Its Association with Milk Production Traits in Chinese Holstein Cattle (Bos taurus) |
| FENG Xiao-Fang1,2, SHEN Yu-Hua1, PENG Cheng3, SUN Guo-Hu3, GU Ya-Ling2, ZHENG Juan-Shan1, GUO Peng-Hui1, ZHANG Juan2,* |
1 College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, China;
2 College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China;
3 Gansu Provincial General Station of Animal Husbandry Technology Extension, Lanzhou 730030, China |
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Abstract Milk fat and protein percentages are critical indicators of milk quality in dairy cows (Bos taurus). Acyl-CoA synthetase long chain family member 6 (ACSL6) is a key enzyme involved in fatty acid metabolism and is considered a promising candidate gene influencing milk production traits. This study aimed to investigate single nucleotide polymorphism (SNP) in the ACSL6 gene and their association with milk production traits in Chinese Holstein cattle. A total of 1 152 cows with clear pedigrees and complete production records were genotyped. Ten SNPs within the ACSL6 gene were identified through PCR amplification and Sanger sequencing, and subsequently genotyped using Sequenom mass spectrometry. Associations between SNPs and 5 traits—milk fat percentage, milk protein percentage, milk fat yield, milk protein yield, and 305-day milk yield—were analyzed using a general linear model. The result showed that all SNPs were in Hardy-Weinberg equilibrium (P>0.05) and exhibited moderate polymorphism (0.25<PIC<0.5) in the studied population. Association analysis revealed that the SNP locus g.22501195 C>T significantly influenced milk fat percentage (P<0.05), with the CC and TT genotypes exhibiting higher milk fat percentage. The SNP locus g.22502247 T>C showed significant correlation with milk fat percentage (P<0.05), and individuals with CC genotype demonstrated significantly higher milk fat percentage compared to those with TC and TT genotypes. The SNP locus g.22527204 C>T had significant effects on both milk fat percentage and milk protein percentage (P<0.05), with CC genotype individuals exhibiting elevated levels in both traits. The SNP locus g.22532788 A>T significantly influenced milk protein percentage (P<0.05), and AA genotype individuals demonstrated significantly higher milk protein percentage. These 4 loci formed 2 haplotype blocks (Block1 and Block2): Block1 showed significant association with milk fat percentage (P<0.05), while Block2 exhibited significant association with milk protein yield (P<0.05). The results of this study revealed a potential association between ACSL6 gene polymorphisms and milk production traits in Chinese Holstein cattle, suggesting that this gene could serve as an important candidate gene for screening molecular markers associated with high-yield and high-quality milk. This study provides a theoretical basis for molecular breeding and genetic improvement of high-quality milk traits in dairy cows.
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Received: 30 March 2026
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Corresponding Authors:
*zhangjkathy@126.com
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[1] 程菲, 潘坛, 张飞, 等. 2021. 奶山羊ACSL6基因克隆及表达调控分析[J]. 中国畜牧兽医, 48(07): 2291-2301.
(Cheng F, Pan T, Zhang F, et al.2021. Cloning and expression regulation analysis of ACSL6 gene in dairy goats[J]. China Animal Husbandry & Veterinary Medicine, 48(07): 2291-2301.)
[2] 杜云霞, 佘汉, 吴银雨, 等. 2024. 抑制乙酰辅酶A羧化酶2改善脓毒症小鼠心功能障碍的机制研究[J]. 陆军军医大学学报, 46(24): 2689-2697.
(Du Y X, She H, Wu Y Y, et al.2024. Mechanism of inhibiting acetyl-CoA carboxylase 2 in improving cardiac dysfunction in septic mice[J]. Journal of Army Medical University, 46(24): 2689-2697.)
[3] 李宏伟, 徐凌洋, 王泽昭, 等. 2022. 基于单倍型肉牛屠宰性状全基因组关联分析研究[J]. 畜牧兽医学报, 53(12): 4232-4243.
(Li H W, Xu L Y, Wang Z Z, et al.2022. Haplotype-based genome-wide association study for carcass traits in beef cattle[J]. Acta Veterinaria et Zootechnica Sinica, 53(12): 4232-4243.)
[4] 刘辰晖, 吴海龙, 程蕾, 等. 2023. FYN结合蛋白基因rs109262355突变对中国南方荷斯坦牛泌乳性能的影响[J]. 浙江大学学报(农业与生命科学版), 49(02): 261-268.
(Liu C H, Wu H L, Cheng L, et al.2023. Effect of rs109262355 mutation in FYN binding protein gene on lactation performance of Southern Chinese Holstein cows[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 49(02): 261-268.)
[5] 宋书媛. 2020. ACSL6对奶牛乳腺上皮细胞乳脂合成的调控作用研究[D]. 硕士学位论文, 东北农业大学, 导师: 侯晓明, pp. 20-45.
(Song S Y.2020. Study on the regulation of ACSL6 on milk fat synthesis in dairy mammary epithelial cells[D]. Thesis for M.S., Northeast Agricultural University, Supervisor: Hou X M, pp. 20-45.)
[6] 宋雨, 许令娜, 韩博, 等. 2024. 中国荷斯坦牛PRKACA基因对产奶性状的遗传效应分析[J]. 中国畜牧杂志, 60(01): 131-136.
(Song Y, Xu L N, Han B, et al.2024. Genetic effect analysis of PRKACA gene on milk production traits in Chinese Holstein cows[J]. Chinese Journal of Animal Science, 60(01): 131-136.)
[7] 宋志芳, 于国升, 邢荷岩, 等. 2017. 单倍型分析及其在全基因组关联分析中的研究进展[J]. 猪业科学, 34(08): 120-122.
(Song Z F, Yu G S, Xing H Y, et al.2017. Research progress of haplotype analysis and its application in genome-wide association study[J]. Swine Industry Science, 34(08): 120-122.)
[8] 屠道远. 2025. MARCH8介导SREBP1泛素化降解调控脂肪酸从头合成抑制肝癌的机制研究[D]. 博士学位论文, 扬州大学, 导师: 柏斗胜, pp. 50-80.
(Tu D Y.2025. Mechanism of MARCH8-mediated ubiquitination and degradation of SREBP1 in regulating de novo fatty acid synthesis and inhibiting hepatocellular carcinoma[D]. Thesis for Ph.D., Yangzhou University, Supervisor: Bo D S, pp. 50-80.)
[9] 吴涛, 齐云霞, 朱洪龙, 等. 2024. 中国荷斯坦奶牛CSN3基因多态性检测及其与产奶性状的关联分析[J]. 畜牧与兽医, 56(08): 15-20.
(Wu T, Qi Y X, Zhu H L, et al.2024. Polymorphism detection of CSN3 gene and its association with milk production traits in Chinese Holstein cows[J]. Animal Husbandry & Veterinary Medicine, 56(08): 15-20.)
[10] 熊畑畑, 吴钰, 邵钺馨, 等. 2025. 奶山羊ACSL6基因启动子克隆与转录活性分析[J]. 农业生物技术学报, 33(01): 124-133.
(Xiong T T, Wu Y, Shao Y X, et al.2025. Promoter cloning and transcriptional activity analysis of ACSL6 gene in dairy goats[J]. Journal of Agricultural Biotechnology, 33(01): 124-133.)
[11] 杨千钰, 郭子骄, 郑伟杰, 等. 2025. 中国荷斯坦牛SAA3.2基因对产奶性状的遗传效应分析[J]. 中国畜牧杂志, 61(01): 144-149.
(Yang Q Y, Guo Z J, Zheng W J, et al.2025. Genetic effect analysis of SAA3.2 gene on milk production traits in Chinese Holstein cows[J]. Chinese Journal of Animal Science, 61(01): 144-149.)
[12] 周月玲, 达日格日乐, 唐永杰, 等. 2022. 中国荷斯坦牛乳房炎抗性相关SNP效应扩群验证及分析[J]. 中国畜牧杂志, 58(12): 92-98.
(Zhou Y L, Dari G R L, Tang Y J, et al.2022. Validation and analysis of SNP effects related to mastitis resistance in Chinese Holstein cows[J]. Chinese Journal of Animal Science, 58(12): 92-98.)
[13] Castillo M I, Freire E, Romero V I, et al.2022. Novel variation in acyl-CoA synthetase long chain family member 6 (ACSL6) results in protein structural modification and multiple non-related neoplasia in a 46-year-old: Case report[J]. Frontiers in Oncology, 12: 899579.
[14] Fernandez R F, Pereyra A S, Diaz V, et al.2021. Acyl-CoA synthetase 6 is required for brain docosahexaenoic acid retention and neuroprotection during aging[J]. JCI Insight, 6(11): e144351.
[15] Hunt R C, Simhadri V L, Iandoli M, et al.2014. Exposing synonymous mutations[J]. Trends in Genetics, 30(7): 308-321.
[16] Jaiswal L, Worku M.2022. Recent perspective on cow's milk allergy and dairy nutrition[J]. Critical Reviews in Food Science and Nutrition, 62(27): 7503-7517.
[17] Johnsson M.2023. Genomics in animal breeding from the perspectives of matrices and molecules[J]. Hereditas, 160(1): 20.
[18] Jung Y H, Bu S Y.2020. Suppression of long chain acyl-CoA synthetase blocks intracellular fatty acid flux and glucose uptake in skeletal myotubes[J]. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1865(7): 158678.
[19] Lee J, Jung J H, Oh S H.2024. Enhancing animal breeding through quality control in genomic data - a review[J]. Journal of Animal Science and Technology, 66(6): 1099-1108.
[20] Li Z, Lu S, Cui K, et al.2020. Fatty acid biosynthesis and transcriptional regulation of stearoyl-CoA desaturase 1 (SCD1) in buffalo milk[J]. BMC Genetics, 21(1): 23.
[21] Nadeem A, Maryam J.2016. Genetic and genomic dissection of prolactin revealed potential association with milk production traits in riverine buffalo[J]. Tropical Animal Health and Production, 48(6): 1261-1268.
[22] Niculite C, Enciu A, Hinescu M E.2019. CD36: Focus on epigenetic and post-transcriptional regulation[J]. Frontiers in Genetics, 10: 680.
[23] Panigrahi M, Rajawat D, Nayak S S, et al.2025. Advancements in animal breeding: From mendelian genetics to machine learning[J]. International Journal of Molecular Sciences, 26(23): 12345.
[24] Putri M, Syamsunarno M R A A, Iso T, et al.2015. CD36 is indispensable for thermogenesis under conditions of fasting and cold stress[J]. Biochemical and Biophysical Research Communications, 457(4): 520-525.
[25] Sauna Z E, Kimchi-Sarfaty C.2011. Understanding the contribution of synonymous mutations to human disease[J]. Nature Reviews Genetics, 12(10): 683-691.
[26] Shishikura K, Kuroha S, Matsueda S, et al.2019. Acyl-CoA synthetase 6 regulates long-chain polyunsaturated fatty acid composition of membrane phospholipids in spermatids and supports normal spermatogenic processes in mice[J]. The FASEB Journal, 33(12): 14194-14203.
[27] Tian H, Niu H, Luo J, et al.2022. Knockout of stearoyl-CoA desaturase 1 decreased milk fat and unsaturated fatty acid contents of the goat model generated by CRISPR/Cas9[J]. Journal of Agricultural and Food Chemistry, 70(13): 4030-4043.
[28] Wang Y, Yu W, Li S, et al.2022. Acetyl-CoA carboxylases and diseases[J]. Frontiers in Oncology, 12: 836058.
[29] Xu L, Liu Q, Long T, et al.2022. Ectopic ACSL6 overexpression partially improves isoproterenol-induced cardiac hypertrophy in vivo and cardiomyocyte hypertrophy in vitro[J]. Journal of Cardiovascular Pharmacology, 80(6): 792-803.
[30] Xu L, Shi L, Liu L, et al.2019. Analysis of liver proteome and identification of critical proteins affecting milk fat, protein, and lactose metabolism in dairy cattle with iTRAQ[J]. Proteomics, 19(12): e1800387.
[31] Yang Z, Lian Z, Liu G, et al.2021. Identification of genetic markers associated with milk production traits in Chinese Holstein cattle based on post genome-wide association studies[J]. Animal Biotechnology, 32(1): 67-76.
[32] Zhu Y, Chen G, Qi H, et al.2025. Inhibition of ACSL6 ameliorates atherosclerosis by suppressing macrophage ferroptosis via the Nrf2 pathway[J]. Molecular Biology Reports, 53(1): 53. |
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