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| Comparative Study on the Efficiency of Transient Transformation in Phalaenopsis spp. Mediated by Three Distinct Vector |
| MAO Ying, SHI Qiu-Yang, CUI Yong-Yi, XIN Jing-Jing* |
| College of Horticulture, Zhejiang A＆F University, Hangzhou 311300, China |
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Abstract Phalaenopsis spp. suffers from complex genetic backgrounds and difficult in vitro regeneration, compounded by the low efficiency of traditional stable genetic transformation, which hampers research on gene function and molecular breeding. Virus vector-mediated transient transformation technology offers an effective approach to address these issues. In this study, Phalaenopsis 'Zishuijing' was used to compare the transient genetic transformation efficiencies mediated by 3 viral vectors: Cymbidium mosaic virus (CymMV), Tobacco rattle virus (TRV), and Cabbage leaf curl virus (CaLCuV). Recombinant vectors carrying the phytoene desaturase (PDS) gene were introduced into leaves via Agrobacterium-mediated injection. The silencing efficiency of the 3 vectors was evaluated based on the photobleaching phenotype induced by PDS silencing and analysis of gene expression levels. The results indicated that significant differences in performance among the 3 vectors, with pCymMV demonstrating the highest transformation efficiency. Color fading was observed on inoculated leaves 4 weeks post-inoculation, and albinism emerged in newly developed leaves at 7 weeks. Moreover, the expression level of PePDS showed a nearly linear decreasing trend. In conclusion, pCymMV was the optimal vector for mediating transient transformation in Phalaenopsis. This study provides an efficient technical foundation for gene function analysis and molecular breeding in Phalaenopsis.
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Received: 05 January 2026
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Corresponding Authors:
*xinjj@zafu.edu.cn
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