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| Expression Characteristics Analysis of Gonadal Aromatase Gene cyp19a1a in Tissues and During Gonad Development Stage of Hemibagrus guttatus |
| LI Guang-Lue1,2, CHEN Yan-Yun1, FANG Jun-Chao2, HU Dan2, LUO Wen-Yin2, ZHU Jia-Jie1,*, HU Qiao-Mu2,* |
1 Guangxi Key Laboratory of Polysaccharide Materials and Modification, Guangxi Minzu University, Nanning 530006, China;
2 Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China |
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Abstract Hemibagrus guttatus is a rare freshwater fish species, sexual dimorphism is the basis of its reproduction, but the mechanism of sex differentiation in H. guttatus remains unclear. The cytochrome P450 family 19 subfamily A member 1A(cyp19a1a) is a critical gene that influences estrogen biosynthesis and is essential for both sex differentiation and ovarian development. To investigate the mechanism of the cyp19a1a gene in sex differentiation of H. guttatus, qPCR was used to analyze the expression levels of cyp19a1a in different tissues, at different developmental stages, and in the gonads of sex-reversed individuals. Fluorescence in situ hybridization (FISH) was employed to determine the expression of cyp19a1a in gonadal cells of H. guttatus. The results revealed that the ORF sequence of cyp19a1a in H. guttatus was 1 587 bp in length, encoding 528 amino acids, and contained conserved functional domains of the aromatase amino acid sequence. The qPCR results showed that cyp19a1a was expressed in all examined tissues of H. guttatus, with the highest expression levels in the gonads, followed by the brain and liver. The expression profile of cyp19a1a in pseudofemales was consistent with that of normal females at the same age. Moreover, the expression of cyp19a1a in ovaries was significantly higher than that in testes at corresponding stages (P<0.05) and gradually increased with gonadal development. FISH results showed that the signal for cyp19a1a was weaker in sperm and mature oocytes but stronger in oogonia and early oocytes. The above findings indicated that the cyp19a1a gene played an important role in gonadal development, germ cell growth, and sex reversal in H. guttatus. This study provides a reference for understanding the function of the cyp19a1a gene and the sex determination mechanisms in H. guttatus.
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Received: 25 August 2025
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Corresponding Authors:
* 20220190@gxmzu.edu.cn; hqmu0806@163.com
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