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| Screening, Identification and Fermentation Optimization of A Strain for Promoting Seed Germination of Cistanche deserticola |
| ZHU Qing-Yue1, WANG Xue-Jing2, CHEN Yong2, WANG Ze-Yu3, BAO Xiao-Wei1,*, SONG Su-Qin2,* |
1 College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China;
2 Urumqi Comprehensive Experimental Station, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences,Urumqi 830000, China;
3 Agricultural Resources and Environment Research Institute, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 831814, China |
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Abstract Cistanche deserticola, an obligate root parasitic plant of the Cistanche genus in the family Liliaceae,, possesses significant medicinal value. However, its industrial cultivation and sustainable development are severely constrained by the difficulty of seed germination. This study aims to screen functional strains enhancing seed germination in C. deserticola and optimize fermentation conditions to boost the yield of bioactive metabolites. Initial screening was performed using culture plates assessing plant growth-promoting traits, followed by secondary screening via seed germination assays of C. deserticola, with target strains subsequently identified. Growth kinetics under saline-alkaline stress were determined in liquid culture, followed by targeted fermentation optimization to maximize indole-3-acetic (IAA) and siderophore activity of the selected strain. A functionally effective strain, NSS1-6, was isolated from the rhizospheric soil of H. ammodendron and the C. deserticola-H. ammodendron parasitic sites. The strain demonstrated biosynthesis of IAA and siderophores, significantly enhancing seed germination (increased from 7.53% to 44.96%) and haustorium formation rates (increased from 3.46% to 26.31%) in C. deserticola. The strain NSS1-6 was identified as Bacillus cereus. It can grow in a liquid medium with a NaCl concentration of 11% and pH of 11, and has a strong ability to tolerate salt and alkali. The optimal fermentation conditions for this strain were determined as follows: Initial pH 7.5, temperature 34.5 ℃, liquid charge of 50%, and inoculum size 3.5% (v/v). Under these conditions, strain NSS1-6 achieved an IAA yield of (52.23±0.22) μg/mL (35.91% increase over pre-optimized levels) and a siderophore activity of (79.73±0.36%)(18.07% enhancement). This study provides theoretical support for deploying plant growth promoting rhizobacteria (PGPR) to enhance seed germination and haustorium formation in Cistanche deserticola, with significant implications for desert cultivation practices.
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Received: 30 May 2025
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Corresponding Authors:
*408531623@qq.com; suqin_song@163.com
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