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| Study on Green Preparation of the Carbon Quantum Dots Derived from Polygonatum cyrtonema and Their Antibacterial Activity Against Pectobacterium carotovorum |
| FENG Jian-Wen1,2, ZHU Yu-Jing1, WANG Qin2, FANG Yang-Hui3, CHEN Mei-Chun4, XIAO Rong-Feng4, SU Hai-Lan1, ZHENG Mei-Xia1,* |
1 Institute of Crop Sciences (Fujian Germplasm Resources Center), Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;
2 School of Life Sciences, Xiamen University, Xiamen 350002, China;
3 Fujian Forestry Science and Technology Extension General Station, Fuzhou 350003, China;
4 Institute of Resources, Environment and Soil Fertilizer, Fujian Academy of Agricultural Sciences, Xiamen 350013, China |
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Abstract Soft rot disease caused by Pectobacterium carotovorum seriously affects the yield and quality of crops and medicinal plants. The development of environmentally friendly green antibacterial agents is essential for the sustainable development of agriculture. In this study, carbon quantum dots derived from Polygonatum cyrtonema (PC-CQDs) were synthesized using the hydrothermal method with waste biomass resources as precursors. The morphology, surface functional groups, charge characteristics, optical properties and thermal stability of PC-CQDs were systematically studied by transmission electron microscopy, X-ray diffraction, dynamic light scattering, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, ultraviolet-visible spectroscopy, fluorescence spectroscopy, and thermogravimetry analysis. Meanwhile, the antibacterial activity of PC-CQDs against P. carotovorum (strain number: FJAT-49333) was evaluated by the inhibition zone method, serial dilution method and toxic plate method with bacteria. The results showed that PC-CQDs had uniform particle size, excellent dispersion, strong hydrophilicity and good thermal stability. PC-CQDs were nearly spherical nanoparticles with obvious graphene lattice structure, and the surface contained hydroxyl, amino, carboxyl, sulfonic acid and amide groups. The average diameter of the was (2.508±0.940) nm, the hydrated particle size could reach (476.2±53.7) nm, and the zeta potential was (-12.09±5.07) mV. Thermal decomposition began above 120 ℃. The inhibition zone diameter of PC-CQDs against FJAT-49333 was (18.085±1.409) mm, and the minimum inhibitory concentration (MIC) was 18.8 mg/mL. When the concentration of PC-CQDs was 1/2 MIC (9.4 mg/mL), it could significantly inhibit the growth and reproduction of FJAT-49333. This study not only provides a feasible method for the biological control of plant soft rot disease, but also offers a way for the high-value utilization of waste biomass resources of P. cyrtonema, and provides a theoretical basis for the development of green antibacterial agents.
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Received: 08 May 2025
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Corresponding Authors:
*zhengmeixia2005@163.com
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