小麦淀粉分支酶SBE基因家族鉴定及<em>TaSBE1</em>-<em>7B</em>功能标记开发

doi:10.3969/j.issn.1674-7968.2026.05.005

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Abstract Starch branching enzyme (SBE) is a type of glycosyltransferases that has 2 catalytic functions, it synthesizes and converts amylopectin as part of the starch synthesis pathway. It plays a key regulatory role during grain development in wheat (Triticum aestivum). To investigate the expression characteristics of TaSBE family genes during wheat grain development and identify superior haplotypes, bioinformatics methods were used to identify TaSBE family members across the genome, analyze their expression patterns, and develop and validate functional molecular markers. The results showed that 15 TaSBE genes were found throughout the wheat genome, dispersed on chromosomes 2A, 2B, 2D, 7A, 7B, and 7D. These genes could be divided into 3 subfamilies, with members of the same subfamily sharing comparable gene structures and shared motifs. Intraspecific synteny analysis revealed 9 pairs of chromosomes with intragroup synteny, demonstrating the evolutionary conservation of family members. Analysis of cis-acting elements revealed that TaSBE family genes contain a large number of developmental response elements. RNA-seq and qPCR analysis revealed that the TaSBE1-7A, TaSBE1-7B, and TaSBE1-7D genes were highly expressed in grains. Sequence polymorphism analysis revealed the presence of regular SNP sites in the sequences of TaSBE1-7A and TaSBE1-7B, allowing them to be classified into 2 haplotypes. KASP molecular markers were created for TaSBE1-7A and TaSBE1-7B. The results demonstrated that the KASP marker, positioned at 1 088 bp (G/A) in the TaSBE1-7B complete genome sequence, efficiently discriminated the 2 haplotypes. Associative analysis of grain starch content revealed that varieties with the TaSBE1-7B-HapⅠ haplotype had considerably higher grain starch content than those with TaSBE1-7B-HapⅡ, and TaSBE1-7B-HapⅠ was under positive selection during wheat breeding. This study provides a theoretical foundation for further investigation into the molecular processes by which the TaSBE1 gene controls grain growth and starch synthesis.

Key words： Wheat Starch branching enzyme (SBE) Amylopectin Haplotype analysis

Received: 02 December 2025

ZTFLH:

S512.1

Corresponding Authors: ^*yangdl@gsau.edu.cn

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	LI Kang-Chun
	MA Xu-Guang
	GUO Li-Jian
	TIAN Tian
	ZHANG Xian-Feng
	WANG Qiao-Yun
	YANG De-Long

Cite this article:

LI Kang-Chun,MA Xu-Guang,GUO Li-Jian, et al. Identification of SBE Gene Family and Development of Functional Markers for TaSBE1-7B in Wheat (Triticum aestivum)[J]. 农业生物技术学报, 2026, 34(5): 971-983.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2026.05.005 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2026/V34/I5/971

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