小麦黑芒性状的遗传分析与基因定位

doi:10.3969/j.issn.1674-7968.2026.05.003

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (6808 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract In common wheat (Triticum aestivum), the black awn trait is relatively rare, and its genetic mechanism remains unclear. This study aims to genetically map the black awn trait in wheat, laying the foundation for subsequent gene cloning and breeding applications. Analysis of the F_2∶9 RIL population derived from 'Yang16G216' (white awn)/ 'Yang16M6393' (black awn) revealed a segregation ratio of white to black awns consistent with 3∶1. Further bulked segregant exome sequencing (BSE-Seq) was performed on the F_2∶5 RIL population from the cross 'Yang16C106' (white awn)/'Yang20M5623' (black awn). Among 86 566 single nucleotide polymorphism (SNP) sites, 456 ones were highly significantly associated with the black awn trait (at the 99% confidence level). Of these, 99.34% (453) were densely distributed in the distal end of chromosome 1AS (65) and the peri-centromeric region of chromosome 1BL (388), demonstrating that the trait was co-regulated by these 2 loci. Fine mapping localized the chromosome 1AS locus to the 0~8.1 Mb interval, and the chromosome 1BL locus to the 360.4~399.5 Mb interval, which was found to be closely linked with the stripe rust resistance gene Yr26. By breaking this linkage and combining multi-disease resistance gene detection, 3 white-awn lines were selected from the F_2∶5 RIL population, which pyramided the stripe rust resistance gene Yr26, powdery mildew resistance gene Pm21, leaf rust resistance gene LrYang16G216, and Fusarium head blight resistance gene Fhb1. This study provides both theoretical and material foundations for map-based cloning of the black awn gene, as well as for pyramiding multiple disease resistances and molecular breeding applications.

Key words： Wheat Black awn Bulked segregant exome sequencing (BSE-Seq) Gene mapping Yr26 Gene pyramiding

Received: 05 December 2025

ZTFLH:

S512.1

Corresponding Authors: ^*zhaorh86@163.com; fangzhengwu88@163.com

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LI Jun-Mei
	CHEN Tian-Tian
	WAN Wen-Tao
	WANG Ling
	WEI Lu-Yang
	BIE Tong-De
	ZHAO Ren-Hui
	FANG Zheng-Wu

Cite this article:

LI Jun-Mei,CHEN Tian-Tian,WAN Wen-Tao, et al. Genetic Analysis and Gene Mapping of the Black Awn Trait in Wheat (Triticum aestivum)[J]. 农业生物技术学报, 2026, 34(5): 944-954.

URL:

https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2026.05.003 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2026/V34/I5/944

[1] 蔡文华, 裴建文. 1986. 小麦芒对产量构成因素的影响[J]. 甘肃农业科技, (05): 5-7.
(Cai W H, Pei J W. 1986. Effects of wheat awns on yield components[J]. Gansu Agricultural Science and Technology, (05): 5-7.)
[2] 陈玉玉, 张平平, 张保龙, 等. 2025. 基于BSE-seq测序的小麦条锈病高温成株期抗性基因Yr52连锁标记的开发[J]. 植物保护, 51(03): 144-152+181.
(Chen Y Y, Zhang P P, Zhang B L, et al.2025. Development of linkage markers for high-temperature adult-plant resistance gene Yr52 in wheat based on BSE-seq[J]. Plant Protection, 51(03): 144-152, 181.)
[3] 程顺和, 郭文善, 王龙俊, 等. 2012. 中国南方小麦[M]. 南京:江苏科学技术出版社, pp. 188-189.
(Cheng S H, Guo W S, Wang L J, et al.2012. Wheat in Southern China [M]. Jiangsu Science and Technology Press, Nanjing, China, pp. 188-189.)
[4] 谷雨杰. 2024. 小麦黑色颖壳基因的精细定位[D]. 硕士学位论文, 山西农业大学, 导师: 张晓军, pp. 17-18.
(Gu Y J.2024. Fine mapping of the black glume gene in wheat [D]. Thesis for M.S., Shanxi Agricultural University, Supervisor: Zhang X J, pp. 17-18.)
[5] 韩德俊, 王琪琳, 张立, 等. 2010. “西北-华北-长江中下游”条锈病流行区系当前小麦品种(系)抗条锈病性评价[J]. 中国农业科学, 43(14): 2889-2896.
(Han D J, Wang Q L, Zhang L, et al.2010. Evaluation of resistance of current wheat cultivars to stripe rust in Northwest China, North China and the Middle and Lower reaches of Changjiang river epidemic area[J]. Scientia Agricultura Sinica, 43(14): 2889-2896.)
[6] 金迪, 王冬至, 王焕雪, 等. 2019. 小麦芒长抑制基因B2的精细定位与候选基因分析[J]. 作物学报, 45(6): 807-817.
(Jin D, Wang D Z, Wang H X, et al.2019. Fine mapping and candidate gene analysis of awn inhibiting gene B2 in common wheat[J]. Acta Agronomica Sinica, 45(6): 807-817.)
[7] 刘瑞珊. 2025. 野生二粒小麦抗白粉病基因PmLF540的精细定位[D]. 硕士学位论文, 烟台大学, 导师: 马朋涛, pp. 65-66.
(Liu R S.2025. Fine mapping of a powdery mildew resistance gene PmLF540 from wild emmer wheat[D]. Thesis for M.S., Yantai University, Supervisor: Ma P T, pp. 65-66.)
[8] 徐云碧, 杨泉女, 郑洪建, 等. 2020. 靶向测序基因型检测(GBTS)技术及其应用[J]. 中国农业科学, 53(15): 2983-3004.
(Xu Y B, Yang Q N, Zheng H J, et al.2020. Genotyping by target sequencing (GBTS) and its applications[J]. Scientia Agricultura Sinica, 53(15): 2983-3004.)
[9] 朱展望, 徐登安, 程顺和, 等. 2018. 中国小麦品种抗赤霉病基因Fhb1的鉴定与溯源[J]. 作物学报, 44(04): 473-482.
(Zhu Z W, Xu D A, Cheng S H, et al.2018. Characterization of Fusarium head blight resistance gene Fhb1 and its putative ancestor in Chinese wheat germplasm[J]. Acta Agronomica Sinica, 44(04): 473-482.)
[10] Bie T D, Zhao R H, Zhu S Y, et al.2015. Development and characterization of marker MBH1 simultaneously tagging genes Pm21 and PmV conferring resistance to powdery mildew in wheat[J]. Molecular Breeding, 35: 189.
[11] Biffen R H.1905. Mendel's laws of inheritance and wheat breeding[J]. The Journal of Agricultural Science, 1(1): 4-48.
[12] Chen P D, Qi L L, Zhou B, et al.1995. Development and molecular cytogenetic analysis of wheat-Haynaldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew[J]. Theoretical and Applied Genetics, 91(6): 1125-1128.
[13] Chen, Y M, Kong D H, Wang, Z K, et al.2025. A wheat CC-NBS-LRR protein Ym1 confers WYMV resistance by recognizing viral coat protein[J]. Nature Communications, 16(1): 3630.
[14] Deng X, Zhen S M, Liu D M, et al.2019. Integrated proteome analyses of wheat glume and awn reveal central drought response proteins under water deficit conditions[J]. Journal of Plant Physiology, 232: 270-283.
[15] Dong C H, Zhang L C, Chen Z X, et al.2020. Combining a new exome capture panel with an effective varBScore algorithm accelerates BSA-based gene cloning in wheat[J]. Frontiers in Plant Science, 11: 1249.
[16] Grundbacher F J.1963. The physiological function of the cereal awn[J]. Botanical Review, 29(3): 366-381.
[17] Huang D Q, Zheng Q, Melchkart T, et al.2020. Dominant inhibition of awn development by a putativezinc-finger transcriptional repressor expressed at the B1 locus in wheat[J]. New Phytologist, 225(1): 340-355.
[18] Jiao C Z, Xie X M, Hao C Y, et al.2025. Pan-genome bridges wheat structural variations with habitat and breeding[J]. Nature, 637(8045): 384-393.
[19] Khlestkina E K.2013. Genes determining the coloration of different organs in wheat[J]. Russian Journal of Genetics, 3(1): 54-65.
[20] Khlestkina E K, Pshenichnikova T A, Röder M S, et al.2006. Comparative mapping of genes for glume colouration and pubescence in hexaploid wheat (Triticum aestivum L.)[J]. Theoretical and Applied Genetics, 113(5): 801-807.
[21] Khlëstkina E K, Salina E A, Pshenichnikova T A, et al.2000. Analysis of isogenic lines of the soft wheat carrying dominant alleles of Bg, Hg, and Rg1 genes using microsatellite and protein markers[J]. Genetika, 36(10): 1374-1379.
[22] Khlestkina E K, Salina E A, Pshenichnikova T A, et al.2009. Glume coloration in wheat: Allelism test, consensus mapping and its association with specific microsatellite allele[J]. Cereal Research Communications, 37(1): 37-43.
[23] Lang J, Fu Y X, Zhou Y, et al.2021. Myb10‐D confers PHS‐3D resistance to pre‐harvest sprouting by regulating NCED in ABA biosynthesis pathway of wheat[J]. New Phytologist, 230(5): 1940-1952.
[24] Ma J X, Zhou R H, Dong Y S, et al.2001. Molecular mapping and detection of the yellow rust resistance gene Yr26 in wheat transferred from Triticum turgidum L. using microsatellite markers[J]. Euphytica, 120(2): 219-226.
[25] Mao W W, Wang X B, Chen Y M, et al.2025. Map-based cloning and characterization reveal that an R2R3 MYB gene confers red glume in wheat[J]. The Crop Journal, 13: 887-899.
[26] Mascher M, Matthias J, Kuon J E, et al.2014. Mapping-by-sequencing accelerates forward genetics in barley[J]. Genome Biology, 15(6): R78.
[27] Maydup, M L, Antonietta M, Guiamet J J, et al.2010. The contribution of ear photosynthesis to grain filling in bread wheat (Triticum aestivum L.)[J]. Field Crops Research, 119(1): 48-58.
[28] Olugbemi, L B.1978. Distribution of carbon-14 assimilated by wheat awns[J]. Annals of Applied Biology, 90(1): 111-114.
[29] Peleg Z, Saranga Y, Fahima T, et al.2010. Genetic control over silica deposition in wheat awns[J]. Physiologia Plantarum, 140(1): 10-20.
[30] Pshenichnikova T A, Bokarev I E, Shchukina L V.2005. Hybrid and monosomic analyses of smoky coloration of the ear in common wheat[J]. Russian Journal of Genetics, 41(8): 941-943.
[31] Sikka S M, Jain K B, Parmar K S.1961. Inheritance of some morphological characters in intervarietal crosses of Triticum aestivum L[J]. The Journal of the Indian Botanical Society, 40: 217-233.
[32] Wang C M, Zhang Y P, Han D J, et al.2008. SSR and STS markers for wheat stripe rust resistance gene Yr26[J]. Euphytica, 159(3): 359-366.
[33] Zhao R H, Liu B L, Wan W T, et al.2023. Mapping and characterization of a novel adult-plant leaf rust resistance gene LrYang16G216 via bulked segregant analysis and conventional linkage method[J]. Theoretical and Applied Genetics, 136(1): 1.
[34] Zhu T T, Wang L, Rimbert H, et al.2021. Optical maps refine the bread wheat Triticum aestivum cv. Chinese Spring genome assembly[J]. The Plant Journal, 107(1): 303-314.
[35] Zou C, Wang P X, Xu, Y B.2016. Bulked sample analysis in genetics, genomics and crop improvement[J]. Plant Biotechnology Journal, 14(10): 1941-1955.