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| Establishment and Preliminary Application of Determination Methods for Three Small Molecule Metabolites in Complex Yeast Cultures |
| YANG Lan1,*, ZHANG Xiao-Jie1,*, LIAN Hai-Fei2, LIU Da-Cheng1,** |
1 College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
2 Inner Mongolia Agricultural and Animal Husbandry Quality and Safety and Testing Research Institute/Inner Mongolia Agricultural and Animal Husbandry Science and Technology Achievement Transfer and Transformation Center, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010018, China |
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Abstract Accurate quantification of active components in complex yeast culture (CYC) is essential for evaluating its quality and functionality. This study established quantitative analytical methods for 3 key small molecule metabolites—N-acetylglutamic acid (NAG), arachidonic acid (ARA), and taurine (Tau)—which were previously identified through non-targeted metabolomics by this research group. To address the complex matrix of CYC, specific methods were developed: Solid-phase extraction combined with high-performance liquid chromatography was used for the highly polar NAG; dansyl chloride pre-column derivatization followed by high-performance liquid chromatography was applied for the zwitterionic Tau; and methylation derivatization coupled with gas chromatography-flame ionization detection was employed for the lipid-soluble ARA. The results demonstrated good linearity (R2>0.999) for all 3 methods, with precision (relative standard deviation<2%), repeatability (relative standard deviation<5%), and spike recovery rates (81.87%~106.95%) all meeting analytical requirements. The developed methods were applied to analyze a laboratory-prepared CYC sample, revealing contents of NAG, ARA, and Tau as (253.80±8.41), (19.72±0.26), and (230.51±4.69) mg/kg (relative standard deviation≤3.31%), respectively. Significant variations were observed in commercially available yeast culture products, with concentration ranges of 38.28~502.31 mg/kg for NAG, 2.60~73.36 mg/kg for ARA, and 28.11~146.44 mg/kg for Tau. The established methods were accurate, reproducible, and suitable for routine quality monitoring of NAG, ARA, and Tau in CYC, providing a reliable analytical foundation for standardizing applications and optimizing production processes.
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Received: 21 July 2025
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Corresponding Authors:
** nmgldc@163.com
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| About author:: *These authors contributed equally to this work |
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