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| Identification, Expression and Interacting Protein Analysis of WOX Gene Family in Phalaenopsis equestris |
| ZHANG Ting, SHI Zi-Hao, ZHENG Tian-Tian, YU Xin, XU Yan, YI Qing-Ping, ZHU De-Yan* |
| College of Food and Biology/Hubei Engineering Research Center for Specialty Flowers Biological Breeding, Jingchu University of Technology, Jingmen 448000, China |
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Abstract WOX (WUSCHEL-related homeobox) is a plant-specific transcription factor family with important regulatory roles in plant growth and stress adaptation. Phalaenopsis equestris is a widely cultivated flower and of great economic importance in orchids. The identification and analysis of WOX family members is helpful to speculate its function in P. equestris development. Ten members of PeWOX family were characterized by bioinformatics analysis in this study, which were low hydrophilic proteins and most were localized in the nucleus. Phylogenetic analysis showed that PeWOX13A and PeWOX13B belonged to ancient clade, PeWOX9, PeWOX11, and PeWOX12 belonged to intermediate clade, and others belonged to modern clade. Members of 3 clades showed obvious differences in protein structure. Expression profiling of PeWOX genes by qRT-PCR revealed their tissue-specific expression, suggesting that they might have functionally differentiation and were responsible for different organ development. Promoter analysis and the response of PeWOX genes to hormones and stresses by qRT-PCR suggested that PeWOX members were involved in the hormonal and stress response pathways. Interaction protein analysis revealed interaction relationships between PeWOX proteins and other transcription factors, epigenetic modifiers, and kinases, indicating that PeWOXs might have complex regulatory mechanisms. This study provides valuable information for further exploration of PeWOX function.
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Received: 26 November 2024
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Corresponding Authors:
*zhudeyan@jcut.edu.cn
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