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| Genome-wide Identification and Expression Analysis of the PYL Gene Family in Malus sieversii |
| FANG Zhen, LI Jing, ZHANG Kai, MA Juan, YE Chun-Xiu* |
| College of Forestry and Landscape Architecture, Xinjiang Agricultural University, Urumqi 830052, China |
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Abstract The plant hormone abscisic acid (ABA) was the key hormone in the regulation of plant growth and stress response, and played a key role in the regulation of seed dormancy and germination and growth and development, and the ABA receptor pyrabactin resistance (PYR) / PYR1-like (pyrabactin resistance-like, PYL) proteins (PYLs) / regulatory components of ABA receptor (RCAR), as one of the core components of the ABA signaling pathway, plays an important function in the ABA signaling pathway. In this study, bioinformatics methods and transcriptome sequencing were used for genome-wide identification of PYL family members in Malus sieversii, the chromosomal locations, evolutionary relationships, gene structures, protein motifs, covariate and cis regulatory elements were analyzed by bioinformatics methods were used for genome-wide identification of PYL family members in M. sieversii, as well as the expression patterns in different organs, and expression profiles characterized by the low-temperature stratification process of seeds of PYL members by transcriptome sequencing. The results showed that 14 PYL genes were identified in the whole genome of M.sieversii, which were distributed on 7 chromosomes with clustered gene distribution. Phylogenetic analysis classified them into 5 subgroups: groups A~E. The family members contained 0~4 introns, and the encoded proteins of the 14 PYL genes were identified to have PYR_PYL_RCAR_like conserved structures. Cis-acting element analysis showed that a variable number and variety of cis-acting elements involved in hormone response, growth and developmental response, abiotic and biotic stress response were prevalent in the 2 000 bp upstream sequence of the PYL gene in M. sieversii. Gene ontology enrichment showed that the MsiPYL gene mainly played a role in abscisic acid activation signaling pathway, regulation of phosphatase activity and phosphoprotein phosphatase activity, etc. KEGG pathway analysis showed that it was mainly involved in mitogen-activated protein kinase (MAPK) signaling pathway-plant and phytohormone signaling. The MsiPYL genes were constitutively expressed in 6 different tissues, with levels of expression in reproductive organs (flowers and fruit) significantly higher than those in nutrient organs (roots and stems). The results of RNA-seq and qRT-PCR showed that MsiPYL8 (MsiPYR1) had the lowest expression in seeds, which was down-regulated under the low-temperature stratification of M. sieversii seeds, and it might be an important candidate gene in response to seed germination in M. sieversii, which provides a basis for revealing the roles of MsiPYL genes in the release of seed dormancy in M. sieversii.
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Received: 23 July 2024
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Corresponding Authors:
* yecx2008@163.com
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