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| Identification and Expression Analysis of CNGC Gene Family in Pepper (Capsicum annuum) |
| WANG Tian-Tian, CHANG Xue-Rui, WANG Jing* |
| College of Horticulture, Shanxi Agricultural University, Taigu 030801, China |
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Abstract Plant cyclic nucleotide-gated ion channel (CNGC) plays an important role in plant growth, development and stress response. In order to investigate the function of CNGC gene family in pepper (Capsicum annuum), the CNGC family members of pepper were identified, and the physical and chemical properties, subcellular localization, phylogenetic development, structural characteristics, promoter cis-acting elements and gene expression patterns were analyzed. The results showed that a total of 14 CaCNGC gene family members were identified in pepper, which were not uniformly distributed on each chromosome. The isoelectric point of CaCNGC was 8.64~9.61, the molecular weight was 72.87~91.49 kD. The instability index was greater than 40, indicating that 14 members of CaCNGC were unstable proteins. All CaCNGC proteins were located in the plasma membrane, had transmembrane structures, and were hydrophilic proteins. The analysis of cis-element in promoter showed that, most of the CaCNGC contained cis-elements related to growth and development, hormone regulation, and abiotic stresses response. Transcriptome data analysis showed that most members of CaCNGC had low or no expression in leaves, flowers and fruits of pepper. Most members of CaCNGC were expressed under low temperature, high temperature, abscisic acid, gibberellin, hydrogen peroxide, indoleacetic acid, jasmonic acid and salt stress. qRT-PCR analysis showed that the expression of CaCNGC2, CaCNGC7 and CaCNGC10, was up-regulated under low and high temperature stress, which might be involved in the response of pepper to temperature stress. These results provide a theoretical reference for further investigating the function of CaCNGC gene family, and new insights into the molecular mechanisms of pepper tolerance to temperature stress.
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Received: 29 February 2024
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Corresponding Authors:
* wangjing315@sxau.edu.cn
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