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| Development and Application of Molecular Marker for Grain Length Gene GW7 in Rice (Oryza sativa) |
| ZHENG Guo-Li1, GU Qiao-Mei1, DU Ming1, FANG Yu1,2, WANG Pan1,* |
1 Shanghai ZKW Molecular Breeding Technology Co., Ltd., Shanghai 200030, China;
2 Anhui Win-all Hi-tech Seed Co., Ltd., Hefei 230088, China |
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Abstract Rice (Oryza sativa) grain length is an important agronomic trait, which is closely related to rice yield and appearance quality. In order to further explore new molecular markers for improving rice grain length, this study developed a fluorescent molecular markers of GW7 (Gene ID: 10208) gene based on the single base mutation of GW7 gene in the functional region, combined with penta-primer amplification refractory mutation system (PARMS). Combined with grain length phenotype and PCR product sequencing, the detection results of the marker in 13 rice parent materials showed that the marker could accurately identify different GW7 genotypes. Then, 16 homozygous GW7 genotypes were selected from 94 rice materials by using this marker. The results of marker-assisted selection showed that excellent rice materials with increased grain length could be obtained in F2 generation of 'R91' and 'zhuo20'. The marker this study developed can help to selecte individual plants with target traits at the seedling stage, and perform cross-breeding and backcrossing during the flowering period, without having to wait until maturity and harvest thereby accelerating the breeding process for rice grain quality traits.
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Received: 09 November 2023
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Corresponding Authors:
* wp@zkwbreeding.com
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