|
|
|
| Analysis of Wheat (Triticum aestivum) TaPR1 Gene Promoter and Screening of Its Regulators |
| SHEN Song-Song, WANG Li-Shan, FENG Yan, CUI Zhong-Chi, LIU Yuan-Xia, LIU Da-Qun, WANG Hai-Yan* |
| College of Plant Protection/Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province, Hebei Agricultural University, Baoding 071001, China |
|
|
|
|
Abstract Pathogenesis-related protein 1 (PR1) is an important defense protein in plants, its production and accumulation in plants is one of the important responses to biotic and abiotic stresses. In previous study, TaPR1 was found to be induced by Puccinia triticina (Pt) and participate in wheat defense response to leaf rust. In the early stage of this study, the TaPR1 promoter sequence was successfully obtained by gene cloning and named pTaPR1, among which the 900 bp sequence (pTaPR1900) had strong activity. This study further analyzed the sequence composition and possible regulatory factors of pTaPR1. Preliminary analysis based on the PLACE and PlantCARE databases showed that pTaPR1 contained the core elements including TATA-box, CAAT-box and a large number of light-responsive elements, as well as the ABA-responsive element and the MYB-binding site. Yeast one-hybrid (Y1H) technique was used to screen the transcription factors that might bind to pTaPR1900 involved in regulating TaPR1 gene expression, 12 candidates were obtained. The interaction between 4 candidates including U-box E3 ubiquitin ligase, pollen protein, glycine A3 protein and heavy metal-associated isoprenoid phycobiliprotein with pTaPR1900 were initially verified. Taken together, this study provides theoretical reference for exploring the transcriptional regulatory mechanism of TaPR1.
|
|
Received: 29 May 2023
|
|
|
|
Corresponding Authors:
* ndwanghaiyan@163.com
|
|
|
|
[1] 邱士优. 2016. 拟南芥HECT类泛素蛋白连接酶家族基因表达和功能的初步研究[D]. 硕士学位论文, 福建农林大学, 导师: 缪颖, pp. 58-65.
(Qiu S D.2016. The preliminary study of expression and functions of the hect-type ubiquitin-protein ligases (UPL) gene family in Arabidopsis[D]. Thesis for M.S., Fujian Agriculture and Forestry University, Supervisor: Miu Y, pp. 58-65.)
[2] 王丽珊, 王珅, 王菲, 等. 2022. 小麦TaPR1基因启动子的克隆及启动活性分析[J]. 河北农业大学学报, 45(01): 7-11, 68.
(Wang L S, Wang S, Wang F, et al.2022. Cloning and activity analysis of wheat TaPR1 promoter[J]. Journal of Hebei Agricultural University, 45(1): 7-11, 68.)
[3] 王琪, 许志茹, 陈瑾元, 等. 2019. 杨树重金属相关异戊二烯化植物蛋白(HIPPs)基因的鉴定及表达分析[J]. 植物研究, 39(06): 935-946.
(Wang Q, Xu Z R, Chen J Y, et al.2019. Identification and expression analysis of heavy metal-associated isoprenylated plant proteins (HIPPs) genes in Populus[J]. Bulletin of Botanical Research, 39(6): 935-946.)
[4] 许文骐. 2020. 拟南芥MAPRs和Pollen_Ole_e_1在ER胁迫中的功能研究[D]. 硕士学位论文, 华东师范大学, 导师: 李小方, pp. 149-162.
(Xu W Q.2020. The functions of MAPRs and Pollen Ole e 1 under endoplasmic reticulum stress in Arabidopsis thaliana[D]. Thesis for M.S., East China Normal University, Supervisor: Li X F, pp. 149-462.)
[5] 杨琢玉. 2022. 大豆重金属异戊二烯化相关蛋白(GmHIPP22)基因的克隆及功能分析[D]. 硕士学位论文, 吉林农业大学, 导师: 王丕武, pp. 41.
(Yang Z Y.2022. Cloning and functional analysis of soybean heavy metal isoprenoidation-related protein (GmHIPP22) gene[D]. Thesis for M.S., Jilin Agricultural University, Supervisor: Wang P W, pp. 41.)
[6] 张佳玲, 王景一, 景蕊莲, 等. 2020. 小麦E3泛素连接酶基因TaAIRP2-1B克隆及功能分析[J]. 植物遗传资源学报, 21(5): 1237-1244.
(Zhang J L, Wang J Y, Xi Y J, et al.Cloning and functional analysis of an E3 ubiquitin ligase gene TaAIRP2-1B from wheat[J]. Journal of Plant Genetic Resources, 21(5): 1237-1244.)
[7] Bozbuga R.2020. Expressions of pathogenesis related 1 (PR1) gene in Solanum lycopersicum and influence of salicylic acid exposures on host-Meloidogyne incognita interactions[J]. Doklady Biochemistry and Biophysics, 494(1): 266-269.
[8] Cristina U, Manuel C, Miguel A, et al.2006. Expression of PsGRP1, a novel glycine rich protein gene of Pisum sativum, is induced in developing fruit and seed and by ABA in pistil and root[J]. Planta, 223(6): 1292-1302.
[9] Eric L, Philip N, Philip M, et al.1989. Site-specific mutations alter in vitro factor binding and change promoter expression pattern in transgenic plants[J]. Proceedings of the National Academy of Sciences of the USA, 86(20): 7890-7894.
[10] Ghorbel M, Zribi I, Missaoui K, et al.2021. Differential regulation of the durum wheat pathogenesis-related protein (PR1) by calmodulin TdCaM1.3 protein[J]. Molecular Biology Reports, 48(1): 347-362.
[11] Huang J Z, Wu X Q, Gao Z Y, et al.2021. The ring-type protein BOI negatively regulates the protein level of a CC-NBS-LRR in Arabidopsis[J]. Biochemical and Biophysical Research Communications, 578: 104-109.
[12] Hussain R.2012. WRKY transcription factors involved in PR-1 gene expression in Arabidopsis[D]. Thesis for Ph.D., Leiden University, Supervisor: Memelink J, pp. 128-136.
[13] Liao Y, Zou H F, Wei W, et al.2008. Soybean GmbZIP144, GmbZIP162 and GmbZIP178 genes function as negative regulator of ABA signaling and confer salt and freezing tolerance in transgenic Arabidopsis[J]. Planta, 228(2): 225-240.
[14] Liu T L, Chen T Z, Kan J L, et al.2021. The GhMYB36 transcription factor confers resistance to biotic and abiotic stress by enhancing PR1 gene expression in plants[J]. Plant Biotechnology Journal, 20(4): 722-735.
[15] Manghwar H, Hussain A, Ullah A, et al.2018. Expression analysis of defense related genes in wheat and maize against Bipolaris sorokiniana[J]. Physiological & Molecular Plant Pathology, 103: 36-46.
[16] Neuhaus G, Neuhaus-Url G, Katagiri F, et al.1994. Tissue-specific expression of As-1 in transgenic tobacco[J]. The Plant Cell, 6(6): 827-834.
[17] Ordoñez M E, Germán S E, Kolmer J A.2010. Genetic differentiation within the Puccinia triticina population in South America and comparison with the North American population suggests common ancestry and intercontinental migration[J]. Phytopathology, 100(4): 376-383.
[18] Shim J S, Park S H, Kim J K, et al.2021. The rice glycine-rich protein 3 confers drought tolerance by regulating mRNA stability of ROS scavenging-related genes[J]. Rice, 14(1): 1-19.
[19] Singh S A, Virdi A S, Singh P, et al.2017. A temperature-responsive gene in sorghum encodes a glycine-rich protein that interacts with calmodulin[J]. Biochimie, 137: 115-123.
[20] Song W W, Duan F M, Wang J A, et al.2013. GmPOI gene encoding a Pollen_Ole_e_I conserved domain is involved in response of soybean to various stresses[J]. Biologia Plantarum, 57(1): 85-90.
[21] Van Loon L C, Rep M, Pieterse C M J, et al.2006. Significance of inducible defense-related proteins in infected plants[J]. Annual Review of Phytopathology, 44(1): 135-162.
[22] Wang F, Yuan S T, Wang H Y, et al.2020. TaTLP1 interacts with TaPR1 to contribute to wheat defense responses to leaf rust fungus[J]. PLOS Genetics, 16(7): e1008713
[23] Wei W, Cui M Y, Feng J Y, et al.2018. Ectopic expression of FvWRKY42, a WRKY transcription factor from the diploid woodland strawberry (Fragaria vesca), enhances resistance to powdery mildew, improves osmotic stress resistance, and increases abscisic acid sensitivity in Arabidopsis[J]. Plant Science, 275: 60-74. |
|
|
|
