|
|
|
| Construction and Antibacterial Effect Evaluation of Nano TiO2 Loaded Moxifloxacin Hydrochloride System |
| HUANG Han-Ye, GUO Xue-Ping, XU Jia-Wei, LI Qian-Qian, CAI Xin-Kai, GUAN Xiong, PAN Xiao-Hong* |
| State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops/Key Lab of Ministry of Education for Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
|
|
|
|
Abstract Moxifloxacin hydrochloride (MOX) is a broad-spectrum quinolone drug and is widely used in clinic as a antibacterial agent. In order to explore the effect of nanocarriers on its antibacterial effect, MOX was loaded onto nano titanium dioxide (TiO2) by surface adsorption in this study, and then the chemical characterization, antibacterial activity against Escherichia coli and pathological observation of the nano TiO2-MOX were investigated. Nano TiO2 combined with MOX in the form of physical embedding. When the content of nano TiO2 increased from 0.5 mg to 1.0 mg, the drug loading and the encapsulation rate increased by about 20% and 40% respectively. Nano TiO2 in nano TiO2-MOX could help release the effective components of MOX. The results of the in vitro activity against E. coli showed that the synthesized nano TiO2 had certain antibacterial activity (22.8%), and the antibacterial activity of MOX increased from 28.0% to 73.4% with the support of nano TiO2. Pathological observation also indicated that the damage of the cell membrane of E. coli was not obvious by MOX, but nano TiO2 and nano TiO2-MOX could cause obvious damage to the cell membrane and then cause cell death. In this study, the synthesized nano TiO2-MOX showed good drug loading and releasing effect, and significantly improved the antibacterial activity of MOX, which provides a scientific basis for the application of nano TiO2 as a new drug carrier in further clinical research.
|
|
Received: 24 March 2023
|
|
|
|
Corresponding Authors:
*panxiaohong@163.com
|
|
|
|
[1] 杜明权, 李东, 阳宇, 等. 2008. 紫外分光光度法测定盐酸莫西沙星片的含量[J]. 中国现代药物应用, 2(2): 6-7.
(Du M Q, Li D, Yang Y, et al.2008. Determination of moxifloxacin chloride tablets by ultraviolet spectrophotometry[J].Chinese Journal of Modern Drug Application, 2(2): 6-7.)
[2] 胡汉祥, 丘克强. 2006. 激光粒度分析结果在形貌分析中的应用[J]. 理化检验(物理分册), 42(02): 72-74.
(Hu H X, Qiu K Q.2006. Application of laser particle size analysis in morphology analysis[J]. Physical Testing and Chemical Analysis (PartA: Physical testing), 42(02): 72-74.)
[3] 雷勇胜, 丁文宇, 雷圆圆, 等. 2019. 盐酸莫西沙星的波谱分析与晶型分析[J]. 中南药学, 17(11): 1824-1829.
(Lei Y S, Ding W Y, Lei Y Y, et al.2019. Spectral analysis and crystal form analysis of moxifloxacin hydrochloride[J]. Central South Pharmacy, 17(11): 1824-1829.)
[4] 李超, 王明琼, 赵永攀, 等. 2022. 喹诺酮类药物耐药机制研究进展[J]. 动物医学进展, 43(2): 112-115.
(Li C, Wang M Q, Zhao Y P, et al.2022. Research progress in the mechanism of quinolone resistance[J]. Progress In Veterinary Medicine, 43(2): 112-115.)
[5] 李德贵. 2017. 盐酸莫西沙星的合成及结构解析[J]. 华南理工大学学报, 45(2): 83-89.
(Li D G.2017. Synthesis and structure analysis of moxifloxacin hydrochloride[J]. Journal of South China University of Technology, 45(2): 83-89.)
[6] 王希, 汤阳, 张小龙, 等. 2022. 介孔TiO2纳米粒在声动力学治疗肝细胞肝癌应用的生物相容性评估[J]. 复旦学报(医学版), 49(4): 475-483.
(Wang X,Tang Y, Zhang X L, et al.2022. Biocompatibility assessment of mesoporous TiO2 nanoparticles for the application of sonodynamic treatment of hepatocellular cancer[J]. Fudan University Journal of Medical Sciences, 49(4): 475-483.)
[7] 王艳丽, 谈顺, 吴秋霞, 等. 2010. 二氧化钛纳米管作为肿瘤药物载体系统的体外负载及释放[J]. 上海大学学报(自然科学版), 16(6): 582-586+596.(Wang Y L, Tan S, Wu Q X, et al. 2010. Loading and release of titanium dioxide nanotubes as cancer drug delivery system[J]. Journal of Shanghai University (Natural Science Edition), 16(6): 582-586, 596.)
[8] 张嘉明, 张洪光, 吴瑞, 等. 2018. 二氧化钛纳米棒的制备及抗菌性能研究[J]. 中国无机分析化学,8(6): 68-70.
(Zhang J M, Zhang H G, Wu R,et al.2018. Preparation of TiO2 nanorods and its antibacterial property[J]. Chinese Journal of Inorganic Analytical Chemistry, 8(6): 68-70.)
[9] 张新雨, 冯亚婵, 张皓杰, 等. 2022. 纳米载药体系在肿瘤耐药治疗中的应用研究进展[J]. 河北科技大学学报, 43(4): 417-425.
(Zhang X Y, Feng Y C, Zhang H J, et al.2022. Research progress in the application of nano drug delivery system in the treatment of tumor drug resistance[J]. Journal of Hebei University of Science and Technology, 43(4): 417-425.)
[10] 周闯, 张利, 李普旺, 等. 2022. 双改性壳聚糖载药纳米胶束的制备及其性能研究[J]. 高分子通报, 35(10): 99-107.
(Zhou C, Zhang L, Li P W, et al.2022. Preparation and properties of dual modified chitosan based drug-loaded nano-micelles[J]. Polymer Bulletin, 35(10): 99-107.)
[11] 周毛毛, 蒋阳, 谢于辉, 等. 2022. 纳米二氧化钛的制备、改性及其在聚合物基复合材料中的应用研究进展[J]. 复合材料学报, 39(05): 2089-2105.
(Zhou M M, Jiang Y, Xie Y H, et al.2022. Preparation and modification of nano-TiO2 and its application in polymer matrix composites research progress[J]. Acta Materiae Compositae Sinica, 39(05): 2089-2105.)
[12] Abd El-Hamid S M, El-Demerdash R S, Arafat H F H, et al.2017. Spectroscopic studies and thermal analysis of mononuclear metal complexes with moxifloxacin and 2,2 '-bipyridine and their effects on acute lung injury induced by hydrochloric acid in rats[J]. Journal of Molecular Structure, 1149: 613-625.
[13] Afzal O, Altamimi A S A, Nadeem M S, et al.2022. Nanoparticles in drug delivery: From history to therapeutic applications[J]. Nanomaterials, 12(24): 27-31.
[14] Bischoff N S, de Kok T M, Sijm D, et al.2021. Possible adverse effects of food additive E171 (titanium dioxide) related to particle specific human toxicity, including the immune system[J]. International Journal of Molecular Sciences, 22(1): 35-41.
[15] Cazan C, Enesca A, Andronic L.2021. Synergic effect of TiO2 filler on the mechanical properties of polymer nanocomposites[J]. Polymers, 13(12): 24-29.
[16] Duan J H, Vogt F G, Li X J, et al.2013. Design, characterization, and aerosolization of organic solution advanced spray-dried moxifloxacin and ofloxacin dipalmitoylphosphatidylcholine (DPPC) microparticulate/ nanoparticulate powders for pulmonary inhalation aerosol delivery[J]. International Journal of Nanomedicine, 8(1): 3489-3505.
[17] El-Megharbel S M, Adam M A, Megahed A S, et al.2015. Synthesis and molecular structure of moxifloxacin drug with metal ions as a model drug against some kinds of bacteria and fungi[J]. Russian Journal of General Chemistry, 85(10): 2366-2373.
[18] He X J, Zhang G N, Wang X, et al.2017. Biocompatibility, corrosion resistance and antibacterial activity of TiO2/CuO coating on titanium[J]. Ceramics International, 43(18): 16185-16195.
[19] Irshad M A, Nawaz R, Rehman M U, et al.2021. Synthesis, characterization and advanced sustainable applications of titanium dioxide nanoparticles: A review[J]. Ecotoxicology and Environmental Safety, 212: 14-20.
[20] Li A Q, Khan I N, Khan I U, et al.2021. Gellan gum-based bilayer mucoadhesive films loaded with moxifloxacin hydrochloride and clove oil for possible treatment of periodontitis[J]. Drug Design Development and Therapy, 15: 3937-3952.
[21] Liu Y,Yang T N, Zhang B, et al.2020. Structural insight in the interfacial effect in ferroelectric polymer nanocomposites[J]. Advanced Materials, 32(49): 8-13.
[22] McDaniel J S, Scott L F, Rebeles J, et al.2020. Treatment of corneal infections utilizing an ocular wound chamber[J]. Translational Vision Science & Technology, 9(12): 11-16.
[23] Nguyen T C, Nguyen T D, Vu D T, et al.2020. Modification of titanium dioxide nanoparticles with 3-(trimethoxysilyl) propyl methacrylate silane coupling agent[J]. Journal of Chemistry, 6: 1-10.
[24] Pan X H, Nie D Y, Guo X P, Xu, S. S, et al.2023. Effective control of the tomato wilt pathogen using TiO2 nanoparticles as a green nanopesticide[J]. Environmental Science: Nano, 10(5): 1441-1452.
[25] Schneider K, Zajac D, Sikora M, et al.2015. XAS study of TiO2-based nanomaterials[J]. Radiation Physics and Chemistry, 112: 195-198.
[26] Tao J J, Ma H P, Yuan K P, et al.2020. Modification of 1D TiO2 nanowires with GaOxNy by atomic layer deposition for TiO2@GaOxNy core-shell nanowires with enhanced photoelectrochemical performance[J]. Nanoscale, 12(13): 7159-7173.
[27] Teyrnourinia H, Salavati-Niasari M, Arniri O, et al.2019. Application of green synthesized TiO2/Sb2S3/GQDs nanocomposite as high efficient antibacterial agent against E. coli and Staphylococcus aureus[J]. Materials Science & Engineering C-Materials for Biological Applications, 99: 296-303.
[28] Zhao X X,Yuan Y J, Shao Q, et al.2022. Simultaneous determination of moxifloxacin hydrochloride and dexamethasone sodium phosphate in rabbit ocular tissues and plasma by LC-MS/MS: Application for pharmacokinetics studies[J]. Molecules, 27(22): 13-18.
[29] Ziental D, Czarczynska-Goslinska B, Mlynarczyk D T, et al.2020. Titanium dioxide nanoparticles: Prospects and applications in medicine[J]. Nanomaterials, 10(2): 31-36. |
|
|
|
