Abstract：To identify QTL related with fiber qualities in upland cotton (Gossypium hirsutum L.), a composite cross population was derived from the cross between (Yumian 1 × CRI 35) F1 and (Yumian 1 × Belshinuo) F1. A total of 155 SSR polymorphic primer pairs were obtained by selecting 6 565 primer pairs among upland cotton cultivars, CRI 35, Yumian 1 and Belshinuo. Genetic linkage analysis was conducted on 158 loci, which were obtained from genotyping a composite cross population, [(Yumian 1 × CRI 35) × (Yumian 1 × Belshinuo)] F1, and a genetic linkage map was constructed. The map contained 102 SSR loci and covered a whole length of 1 199.0 cM. Based on multiple QTL mapping method, eleven QTLs for fiber quality traits were identified, including four for fiber length, two for uniformity, and five for fiber strength. Eleven QTLs were mapped on four D-genome chromosomes. The present study shows that different upland cotton cultivars have different alleles of identical QTL for the same fiber quality trait. Composite cross population with three parents can increase the polymorphic ratio of DNA marker, and detect the different alleles of identical QTL in different backgrounds.
