Abstract：Objective: In order to study on the growthy characters of co-cultures in porcine, we established the method for co-culture between preadipocytes from porcine firstly. Method: Differential attachment was used to purify preadipocytes and myogenic satellite cells, then both were mixtured and co-cultured in the ratios of 1:10 as preadipocytes and myogenic satellite cells for controls. Preadipocytes and myogenic satellite cells were identified respectively with Oil Red O and Desmin immunohislochemistry staining. Their morphological changes, MTT, growth curve all described the growthy regularity of co-cultures. Result: The myoblasts purity exceeded to 97% by Desmin immunohislochemistry staining test, and more than 60% of preadipocytes developed to differentiated adipose cells as defined by Oil Red O staining. Before confluence the micrography of typical co-cultures was fusiform shape by survey, but after confluence changed into irregular with coinciding and interlace with each other, the cells filled lipid appear later and fewer than control group. HE staining demonstrated polykaryocytes fuse into myotubes. MTT and growth curves for 8 days show the growthy rate of co-cultures is more than control groups, a rapid growth until 7 days after the delitescence of growth from 2 to 3 days like as the control groups, but keep on slow later growth to 9 days. Conclusion: The method for co-culture of preadipocytes with myogenic satellite cells in porcine is established premilinaryly, and the basic growthy characters of co-cultures are identified.
孙红梅 孙超*. 猪前体脂肪细胞与肌卫星细胞体外联合培养的初步研究[J]. , 2007, 15(4): 0-.
. The preliminary study on the co-culture of preadipocytes and myogenic satellite cells. , 2007, 15(4): 0-.
