Functional Characterization of TaCBL1 in the Interaction Between Wheat (Triticum aestivum) and Puccinia triticina
WANG Meng-Xuan*, NIU Ze-Lin*, WANG Qian, MA Nan, LIU Na, SUN Tian-Jie**, WANG Dong-Mei**
State Key Laboratory of North China Crop Improvement and Regulation/Hebei Province Key Laboratory of Plant Physiology and Molecular Pathology/College of Life Sciences, Hebei Agricultural University, Baoding 071001, China
Abstract：Puccinia triticina (Pt), an obligate fungal pathogen, induces leaf rust on wheat and is a major cause of yield loss. This study investigates the function and underlying mechanisms of the Triticum aestivum calcineurin B-like protein 1 (TaCBL1) in the interaction between wheat (Triticum aestivum) and Pt, aiming to lay the groundwork for a deeper understanding of the calcium signaling pathway governing this interaction. Expression patterns of TaCBL1 were analyzed in transcriptome data from wheat-Pt interactions and validated by qRT-PCR. Functional analyses were performed using virus-induced gene silencing (VIGS), RNA interference (RNAi)- and overexpression (OE) lines to assess TaCBL1 function during the wheat-Pt interaction. The effects of TaCBL1 silencing or overexpression on pathogenesis-related genes (PRs) expression were assessed by qRT-PCR, and H2O2 accumulation was examined using diaminobenzidine (DAB) staining to gain preliminary insight into the underlying mechanisms. Results showed that following inoculation with Pt, TaCBL1 expression in the incompatible combination was significantly elevated at 6 and 12 h (P<0.05), with expression levels markedly higher than those in the compatible combination (P<0.05). Silencing TaCBL1 by VIGS and RNAi increased the number of haustorial mother cells (HMCs) of the Pt relative to controls, whereas TaCBL1 overexpression significantly suppressed fungal development (P<0.05). These findings indicate that TaCBL1 exerts a positive regulatory role in the wheat-Pt interaction, providing new evidence for the involvement of calcium signaling in the regulation of pathogen invasion, providing the foundation for further systematic analysis of the molecular mechanisms by which TaCBL1 positively regulates wheat resistance, and enriching the notion of disease resistance breeding centered on signal transduction.
王梦璇, 牛泽霖, 王茜, 麻楠, 刘娜, 孙天杰, 王冬梅. TaCBL1在小麦与叶锈菌互作过程中的功能研究[J]. 农业生物技术学报, 2026, 34(9): 1825-1840.
WANG Meng-Xuan, NIU Ze-Lin, WANG Qian, MA Nan, LIU Na, SUN Tian-Jie, WANG Dong-Mei. Functional Characterization of TaCBL1 in the Interaction Between Wheat (Triticum aestivum) and Puccinia triticina. 农业生物技术学报, 2026, 34(9): 1825-1840.
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