Abstract：Aeonium arboreum and its cultivars, A. arboreum and its range of succulent cultivars in the genus Aeonium of the Crassulaceae family, are important indoor ornamental plants. The absence of a transformation system in this kind of plant restricts its molecular-level studies. This study utilized the A. arboreum cultivars A. arboreum 'Halloween', A. arboreum 'Velour European' and A. arboreum as materials. Different whorl leaves and different strains of Agrobacterium rhizogenes were used to produce hairy roots under non-tissue cultured conditions, aiming to establish a non-tissue cultured hairy root transformation system for A. arboreum and its cultivars. The results indicated that using intermediate whorl leaves and the Agrobacterium rhizogenes strain ArQual could achieve the highest hairy root induction rates, with hairy root induction rates of 93.33%, 96.67% and 83.33% for A. arboreum 'Halloween', A. arboreum 'Velour European' and A. arboreum, respectively. After infection with ArQual carrying the pBI121 vector, the hairy roots produced by the intermediate whorl leaves of the A. arboreum and its cultivars turned blue upon GUS staining. The transformation efficiency of hairy root from A. arboreum 'Halloween', A. arboreum 'Velour European' and A. arboreum were 89.93%, 82.22% and 69.80%, respectively. The anthocyanin-regulating transcription factor AaMYB113 from A. arboreum 'Halloween' was used to validate the hairy roots transformation system of the A. arboreum and its cultivars, and the results showed that compared with the hairy roots transformed with an empty vector, the hairy roots transformed with AaMYB113 exhibited significantly redder coloration. Both anthocyanin content and the expression levels of anthocyanin biosynthetic structural genes in 'Halloween' hairy roots were significantly increased (P<0.05), demonstrating the feasibility of this transformation system. This study established a non-tissue cultured transformation system of hairy roots in A. arboreum and its cultivars, providing technical support for subsequent functional gene research and molecular breeding in these plants.
赵荣, 韩浩章, 黄思远, 郝雪婷, 汪虞骅, 金朝辉, 张清虹, 李素华, 张丽华. 莲花掌属法师类植物非组培毛状根转化体系的建立与验证[J]. 农业生物技术学报, 2026, 34(4): 895-905.
ZHAO Rong, HAN Hao-Zhang, HUANG Si-Yuan, HAO Xue-Ting, WANG Yu-Hua, JIN Zhao-Hui, ZHANG Qing-Hong, LI Su-Hua, ZHANG Li-Hua. Establishment and Validation of a Non-tissue Cultured Transformation System of Hairy Roots in Aeonium arboreum and Its Cultivars. 农业生物技术学报, 2026, 34(4): 895-905.
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